Purification of a Modified Flagellar Export Apparatus
改良鞭毛出口装置的纯化
基本信息
- 批准号:8394244
- 负责人:
- 金额:$ 2.29万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-08-01 至 2015-07-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAffectAffinityAwardBacterial InfectionsBindingBiochemicalBiological AssayBiological ModelsBiological ProcessBiomedical ResearchBiosensing TechniquesChemotaxisCollaborationsComplementComplexDetergentsDevelopmentDissociationDysenteryEnvironmentEventFilamentFlagellaFundingGenesGeneticGoalsIn VitroInstitutionInterferometryKineticsLiposomesMeasuresMembraneMentorsMethodsMolecularMolecular BiologyOpticsPathogenesisPositioning AttributePreparationProcessProgress ReportsProtein Export PathwayProteinsProtonsPublicationsRecruitment ActivityResearchResearch PersonnelResearch TrainingSalmonella entericaSignal TransductionStructureStudentsSurveysSystemTechniquesTrainingTyphoid FeverUnited States National Institutes of HealthVariantVesicleVirulenceWorkassay developmentcareercell motilityimprovedin vivoinstrumentationkinetosomemeetingsmembernanomachinepathogenic bacteriaprogramsprotein protein interactionreconstitutionretinal rodsstoichiometrysuccesstoolundergraduate research
项目摘要
DESCRIPTION (provided by applicant): The bacterial flagellum is a self-assembling, proton-powered rotary nanomachine that drives motility, allowing chemotaxis. It contains a dedicated type III secretion (T3S) apparatus. Homologous T3S systems are used by pathogenic bacteria to effect virulence, underlying many bacterial diseases such as typhoid fever and bacterial dysentery. Using Salmonella enterica as a model system, we will investigate dynamic interactions among flagellar proteins that allow membrane translocation of export substrates through use of molecular biology, genetics and biochemical techniques including optical biosensing. The specific aims are to complete a kinetic survey of interactions among export apparatus proteins using optical biosensing as the primary tool, to examine higher-than-pairwise interactions that better reflect in vivo dynamics and to further the development of an in vitro T3S assay. Eventual development of an in vitro export assay will be of tremendous significance because it would allow us to examine hypotheses addressing T3SS function currently out of reach. Since the flagellar export apparatus is highly similar to virulence T3S systems, the project will contribute significantly to our understanding of bacterial pathogenesis. AREA program goals of enhancing the research environment and exposing students to meritorious research will be well-served by this project.
描述(申请人提供):细菌鞭毛是一种自组装的、质子动力的旋转纳米机器,它驱动运动,允许趋化性。它包含一个专用的III型分泌物(T3s)装置。病原菌使用同源的T3s系统来产生毒力,导致许多细菌性疾病,如伤寒和细菌性痢疾。以沙门氏菌为模型系统,我们将利用分子生物学、遗传学和包括光学生物传感在内的生化技术来研究鞭毛蛋白之间的动态相互作用,这些鞭毛蛋白允许出口底物的膜转运。其具体目的是以光学生物传感为主要工具完成出口器官蛋白质之间相互作用的动力学调查,检查更好地反映体内动态的高于成对的相互作用,并进一步发展体外T3S分析。体外出口试验的最终发展将具有巨大的意义,因为它将使我们能够检验目前尚不能实现的T3SS功能的假说。由于鞭毛输出装置与毒力T3S系统高度相似,该项目将对我们理解细菌的发病机制做出重大贡献。这个项目将很好地服务于改善研究环境和让学生接触到有价值的研究的区域计划目标。
项目成果
期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JONATHAN L MCMURRY其他文献
JONATHAN L MCMURRY的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JONATHAN L MCMURRY', 18)}}的其他基金
Cell-penetrating peptide adaptors for intracellular cargo delivery
用于细胞内货物递送的细胞穿透肽接头
- 批准号:
10653590 - 财政年份:2019
- 资助金额:
$ 2.29万 - 项目类别:
Cell-Penetrating Peptide-Adaptors for Intracellular Cargo Delivery
用于细胞内货物输送的细胞穿透肽适配器
- 批准号:
9813250 - 财政年份:2019
- 资助金额:
$ 2.29万 - 项目类别:
Cell-penetrating peptide-adaptors for intracellular cargo delivery
用于细胞内货物递送的细胞穿透肽适配器
- 批准号:
9171796 - 财政年份:2016
- 资助金额:
$ 2.29万 - 项目类别:
Purification of a Modified Flagellar Export Apparatus
改良鞭毛出口装置的纯化
- 批准号:
7937382 - 财政年份:2009
- 资助金额:
$ 2.29万 - 项目类别:
Purification of a Modified Flagellar Export Apparatus
改良鞭毛出口装置的纯化
- 批准号:
7916028 - 财政年份:2007
- 资助金额:
$ 2.29万 - 项目类别:
Purification of a Modified Flagellar Export Apparatus
改良鞭毛出口装置的纯化
- 批准号:
7253693 - 财政年份:2007
- 资助金额:
$ 2.29万 - 项目类别:
Purification of a Modified Flagellar Export Apparatus
改良鞭毛出口装置的纯化
- 批准号:
8180151 - 财政年份:2007
- 资助金额:
$ 2.29万 - 项目类别:
Protein Interactions in the Flagellar Export Machinery
鞭毛输出机械中的蛋白质相互作用
- 批准号:
6742064 - 财政年份:2004
- 资助金额:
$ 2.29万 - 项目类别:
Protein Interactions in the Flagellar Export Machinery
鞭毛输出机械中的蛋白质相互作用
- 批准号:
6893385 - 财政年份:2004
- 资助金额:
$ 2.29万 - 项目类别:
INTERHELICAL INTERACTIONS IN THE CANNABINOID RECEPTOR
大麻素受体中的螺旋间相互作用
- 批准号:
6175143 - 财政年份:2000
- 资助金额:
$ 2.29万 - 项目类别:
相似海外基金
How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
- 批准号:
BB/Z514391/1 - 财政年份:2024
- 资助金额:
$ 2.29万 - 项目类别:
Training Grant
BRC-BIO: Establishing Astrangia poculata as a study system to understand how multi-partner symbiotic interactions affect pathogen response in cnidarians
BRC-BIO:建立 Astrangia poculata 作为研究系统,以了解多伙伴共生相互作用如何影响刺胞动物的病原体反应
- 批准号:
2312555 - 财政年份:2024
- 资助金额:
$ 2.29万 - 项目类别:
Standard Grant
RII Track-4:NSF: From the Ground Up to the Air Above Coastal Dunes: How Groundwater and Evaporation Affect the Mechanism of Wind Erosion
RII Track-4:NSF:从地面到沿海沙丘上方的空气:地下水和蒸发如何影响风蚀机制
- 批准号:
2327346 - 财政年份:2024
- 资助金额:
$ 2.29万 - 项目类别:
Standard Grant
Graduating in Austerity: Do Welfare Cuts Affect the Career Path of University Students?
紧缩毕业:福利削减会影响大学生的职业道路吗?
- 批准号:
ES/Z502595/1 - 财政年份:2024
- 资助金额:
$ 2.29万 - 项目类别:
Fellowship
感性個人差指標 Affect-X の構築とビスポークAIサービスの基盤確立
建立个人敏感度指数 Affect-X 并为定制人工智能服务奠定基础
- 批准号:
23K24936 - 财政年份:2024
- 资助金额:
$ 2.29万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Insecure lives and the policy disconnect: How multiple insecurities affect Levelling Up and what joined-up policy can do to help
不安全的生活和政策脱节:多种不安全因素如何影响升级以及联合政策可以提供哪些帮助
- 批准号:
ES/Z000149/1 - 财政年份:2024
- 资助金额:
$ 2.29万 - 项目类别:
Research Grant
How does metal binding affect the function of proteins targeted by a devastating pathogen of cereal crops?
金属结合如何影响谷类作物毁灭性病原体靶向的蛋白质的功能?
- 批准号:
2901648 - 财政年份:2024
- 资助金额:
$ 2.29万 - 项目类别:
Studentship
Investigating how double-negative T cells affect anti-leukemic and GvHD-inducing activities of conventional T cells
研究双阴性 T 细胞如何影响传统 T 细胞的抗白血病和 GvHD 诱导活性
- 批准号:
488039 - 财政年份:2023
- 资助金额:
$ 2.29万 - 项目类别:
Operating Grants
New Tendencies of French Film Theory: Representation, Body, Affect
法国电影理论新动向:再现、身体、情感
- 批准号:
23K00129 - 财政年份:2023
- 资助金额:
$ 2.29万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The Protruding Void: Mystical Affect in Samuel Beckett's Prose
突出的虚空:塞缪尔·贝克特散文中的神秘影响
- 批准号:
2883985 - 财政年份:2023
- 资助金额:
$ 2.29万 - 项目类别:
Studentship