COBRE: NDSU: PROJECT 1: EPIGENETIC REGULATION OF VASOACTIVE INTESTINAL PEPTIDE

COBRE：NDSU：项目 1：血管活性肠肽的表观遗传调控

• 批准号: 8360593
• 负责人: GLENN Paul DORSAM
• 金额: $ 13.69万
• 依托单位: NORTH DAKOTA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-09-23
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360593
• 关键词: Binding; CD4 Positive T Lymphocytes; Cell Proliferation; Centers of Research Excellence; Complex; DNA Binding Domain; Disease; Epigenetic Process; Fetal Development; Funding; G Protein-Coupled Receptor Genes; Genes; Goals; Grant; Hematopoiesis; Heterochromatin; Histone Deacetylase; Histones; Immune; National Center for Research Resources; Nuclear; Nucleosomes; Patients; Peptide Hydrolases; Principal Investigator; Recruitment Activity; Regulation; Research; Research Infrastructure; Resources; Source; Tumor Suppressor Proteins; United States National Institutes of Health; Vasoactive Intestinal Peptide; cost; insight; leukocyte proliferation; metaplastic cell transformation; programs; promoter; transcription factor; vasoactive intestinal peptide receptor 1

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Nearly half of leukemic patients will have deletions in the DNA-binding domain of the Ikaros (IK) gene. The long-term goal of this project is to understand how IK bidirectionally and epigenetically modulates transcriptional expression during cellular transformation as well as during normal hematopoiesis and fetal development. IK, a tumor-suppressor, epigenetic transcription factor, preferentially concentrates in transcriptionally repressive nuclear domains called pericentrimeric heterochromatin (PC-HC) and is co-localized with several complexes, including the transcriptionally repressive nucleosome remodeling complex (NuRD). The central hypothesis is that IK silences vasoactive intestinal peptide receptor-1(VPACR-1) expression by physically binding to IK motifs found in the VPACR-1 promoter that results in the displacement of a crucial Sp1 co-activator. Additionally, IK recruits the repressive HDAC/NuRD complex to the VPACR-1 promoter generating a hypo-acetylated histone profile that further suppresses VPACR-1 regulation in activated CD4 T cells. The significance of this project is to understand leukocyte proliferation and differentiation programs better by gaining a greater insight into the regulatory mechanisms by which IK regulates VPACR-1, a highly expressed GPCR gene expressed in the immune compartment that modulates cellular proliferation and differentiation.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 近一半的白血病患者将在Ikaros（IK）基因的DNA结合结构域中缺失。这个项目的长期目标是了解IK如何双向和表观遗传调节细胞转化过程中的转录表达，以及在正常的造血和胎儿发育。IK是一种肿瘤抑制、表观遗传转录因子，优先集中在称为中心异染色质（pericentrimeric heterochromatin，PC-HC）的转录抑制性核结构域，并与包括转录抑制性核小体重塑复合物（transcriptionally repressive nucleosome remodeling complex，NuRD）在内的几种复合物共定位。 中心假设是IK通过物理结合到VPACR-1启动子中发现的IK基序，导致关键的Sp1辅激活剂的置换，从而沉默血管活性肠肽受体-1（VPACR-1）的表达。此外，IK将抑制性HDAC/NuRD复合物募集到VPACR-1启动子，产生低乙酰化组蛋白谱，其进一步抑制活化的CD 4 T细胞中的VPACR-1调节。 该项目的意义在于通过更深入地了解IK调节VPACR-1的调节机制来更好地理解白细胞增殖和分化程序，VPACR-1是一种在免疫室中表达的高表达GPCR基因，其调节细胞增殖和分化。