ISOLATION AND CHARACTERIZATION OF EXTREMOZYMES FROM ALKALINE LAKES IN NEBRASKA

内布拉斯加州碱性湖泊中极端酶的分离和表征

• 批准号: 8360008
• 负责人: JULIE SHAFFER
• 金额: $ 0.54万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360008
• 关键词: Biotechnology; Cellulases; Communities; Environment; Enzyme Gene; Enzymes; Escherichia coli; Funding; Grant; Lipase; National Center for Research Resources; Nebraska; Peptide Hydrolases; Principal Investigator; Reaction; Research; Research Infrastructure; Resources; Source; Taq Polymerase; Thermus; United States National Institutes of Health; cellulase; cost; enzyme activity; esterase; functional genomics; hyperthermophile; interest; microorganism

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Extreme environments have been of considerable interest in recent years due to the discovery that these "uninhabitable" environments are colonized by a diverse community of microorganisms. These microorganisms have been shown to provide valuable extremozymes, enzymes adapted to catalyze reactions under extreme environmental conditions. One well-known extremozyme is Taq polymerase from the hyperthermophile Thermus aquaticus. This research focuses on the little studied, hypersaline, alkaline lakes in western Nebraska. We will isolate and identify potential microorganisms capable of producing extremozymes of interest for numerous biotechnology applications. We will look for esterases, xylanases, cellulases, proteases, and lipases that are active at high pH's. Microorganisms capable of producing these enzymes will be isolated, the enzyme activity characterized, the enzyme purified, and the enzyme gene sequences identified and cloned into Escherichia coli.

这个子项目是利用这些资源的众多研究子项目之一