ISOLATION AND CHARACTERIZATION OF EXTREMOZYMES FROM ALKALINE LAKES IN NEBRASKA

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 近年来，由于发现这些“不适合居住”的环境被多种多样的微生物群落定殖，极端环境已经引起了相当大的兴趣。 这些微生物已显示出提供有价值的极端酶，即适于在极端环境条件下催化反应的酶。 一种众所周知的极端酶是来自超嗜热菌水生栖热菌的Taq聚合酶。 这项研究的重点是在内布拉斯加州西部研究很少，高盐，碱性湖泊。 我们将分离和鉴定潜在的微生物，这些微生物能够产生许多生物技术应用所感兴趣的极端酶。 我们将寻找酯酶、木聚糖酶、纤维素酶、蛋白酶和脂肪酶，它们在高pH值下具有活性。 将分离能够产生这些酶的微生物，表征酶活性，纯化酶，鉴定酶基因序列并克隆到大肠杆菌中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Extreme environments have been of considerable interest in recent years due to the discovery that these "uninhabitable" environments are colonized by a diverse community of microorganisms. These microorganisms have been shown to provide valuable extremozymes, enzymes adapted to catalyze reactions under extreme environmental conditions. One well-known extremozyme is Taq polymerase from the hyperthermophile Thermus aquaticus. This research focuses on the little studied, hypersaline, alkaline lakes in western Nebraska. We will isolate and identify potential microorganisms capable of producing extremozymes of interest for numerous biotechnology applications. We will look for esterases, xylanases, cellulases, proteases, and lipases that are active at high pH's. Microorganisms capable of producing these enzymes will be isolated, the enzyme activity characterized, the enzyme purified, and the enzyme gene sequences identified and cloned into Escherichia coli.