PROTEOMICS CORE

蛋白质组学核心

• 批准号: 8360050
• 负责人: JOHN B SHABB
• 金额: $ 16.28万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360050
• 关键词: Biomedical Research; Communities; Core Facility; Environment; Fees; Funding; Gel; Grant; Health; Image Analysis; Laboratories; Learning; Mass Spectrum Analysis; National Center for Research Resources; North Dakota; Postdoctoral Fellow; Principal Investigator; Proteins; Proteomics; Research; Research Infrastructure; Research Personnel; Research Project Grants; Research Support; Resources; Services; Source; Students; Technology; Training; Two-Dimensional Gel Electrophoresis; United States National Institutes of Health; biomedical scientist; cost; experience; graduate student; operation; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The major responsibility of the Proteomics Core Facility is to support the research efforts of the INBRE investigators and the research community of the state of North Dakota. The Proteomics Core Facility will consist of an established mass spectrometry laboratory and a protein separation laboratory. The core facility will be directed by Dr. John Shabb who has extensive experience in protein mass spectrometry and proteomics. The core facility manager will be responsible for day-to-day operation of the facility. The core facility will offer the following services at moderate fees: protein separation by two-dimensional gel electrophoresis (2D-PAGE), image analysis of 2D-gels, and protein identification and structural characterization of the separated proteins by mass spectrometry. In addition to the services, the facility will offer educational training courses to undergraduate students, graduate students and post-doctoral fellows within the state. The training courses will include protein separation by 2D-PAGE, 2D-gel image analysis and protein identification by mass spectrometry. Thus, the Proteomics Core Facility will provide opportunities to biomedical scientists and students in North Dakota to employ the cutting edge technologies of proteomics into their research projects as well as to students to learn this important technology in the current biomedical research.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 蛋白质组学核心设施的主要职责是支持 INBRE 研究人员和北达科他州研究界的研究工作。 蛋白质组学核心设施将包括一个已建立的质谱实验室和一个蛋白质分离实验室。 该核心设施将由在蛋白质质谱和蛋白质组学方面拥有丰富经验的 John Shabb 博士领导。 核心设施经理将负责设施的日常运营。 核心设施将以适度的费用提供以下服务：通过二维凝胶电泳 (2D-PAGE) 进行蛋白质分离、2D 凝胶图像分析以及通过质谱法对分离蛋白质进行蛋白质鉴定和结构表征。 除了这些服务外，该设施还将为该州的本科生、研究生和博士后提供教育培训课程。 培训课程将包括通过 2D-PAGE 进行蛋白质分离、2D-凝胶图像分析和通过质谱法进行蛋白质鉴定。 因此，蛋白质组学核心设施将为北达科他州的生物医学科学家和学生提供将蛋白质组学的前沿技术应用到他们的研究项目中的机会，也为学生提供在当前生物医学研究中学习这一重要技术的机会。