CHARACTERIZATION OF KINESIN-3 & CARGO IN TRANSIT WITHIN THE SQUID GIANT AXON

Kinesin-3 的表征

• 批准号: 8360083
• 负责人: Joseph Alan Degiorgis
• 金额: $ 8.28万
• 依托单位: UNIVERSITY OF RHODE ISLAND
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360083
• 关键词: Anatomy; Antibodies; Axon; Axonal Transport; Behavioral Research; Binding; Biomedical Research; Dynein ATPase; Funding; Grant; Kinesin; Mediating; Membrane; Microtubules; Molecular; Motor; Movement; National Center for Research Resources; Organelles; Plus End of the Microtubule; Principal Investigator; Process; Property; Proteins; Research; Research Infrastructure; Resources; Role; Source; Squid; United States National Institutes of Health; axoplasm; cost; particle

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Axonal transport is a process in which membrane bound particles are in transit along microtubules powered by motor proteins belonging to the kinesin and cytoplasmic dynein superfamilies. Recently we identified a Kinesin-3 (KIF1A) that co-purifies with axoplasmic organelles and localizes to organelle/microtubule interfaces. Antibodies raised against Kinesin-3 decorate 100 nm organelles in extruded axoplasm and inhibit organelle transport towards the plus ends of microtubules. These finding suggest that Kinesin-3 may be responsible for moving 100 nm organelles towards the plus ends of microtubules. Here, we propose to characterize the motor properties of purified Kinesin-3 and to identify the molecular anatomy of the Kinesin-3 cargo. Understanding the rate and direction of Kinesin-3 movement and the molecular composition of its cargo will help establish the role of Kinesin-3 in the axon and determine it's contribution to motor mediated transport.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 轴突运输是膜结合颗粒沿着微管运输的过程，微管由属于驱动蛋白和细胞质动力蛋白超家族的运动蛋白提供动力。最近，我们发现了一种与轴浆细胞器共同纯化并定位于细胞器/微管界面的驱动蛋白-3 (KIF1A)。针对 Kinesin-3 的抗体装饰挤出的轴浆中的 100 nm 细胞器，并抑制细胞器向微管正端转运。这些发现表明，Kinesin-3 可能负责将 100 nm 细胞器移向微管的正端。在这里，我们建议表征纯化的 Kinesin-3 的运动特性并确定 Kinesin-3 货物的分子解剖结构。了解 Kinesin-3 运动的速率和方向及其货物的分子组成将有助于确定 Kinesin-3 在轴突中的作用，并确定它对运动介导的运输的贡献。