Neuron death in Parkinson's disease: The role of Trib3

帕金森病中的神经元死亡：Trib3 的作用

• 批准号: 8365298
• 负责人: LLOYD A GREENE
• 金额: $ 35万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-01 至 2017-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365298
• 关键词: Animal Disease Models; Animal Model; Autopsy; Binding; Brain; Cell Culture Techniques; Cell Survival; Cell model; Cell physiology; Cellular Stress; Cessation of life; Development; Disease; Disease Progression; Disease model; Enzymes; Functional disorder; Future; Gene Deletion; Generations; Goals; In Vitro; Indium; Lead; Mediating; Modeling; Molecular; Mus; Nerve Degeneration; Neurons; Parkinson Disease; Patients; Peptides; Phosphorylation; Phosphotransferases; Proteins; Publishing; Role; Scheme; Stress; Symptoms; Testing; Therapeutic; Time; Toxin; Work; alpha synuclein; base; brain tissue; design; disorder control; in vivo Model; inhibitor/antagonist; insight; loss of function; novel strategies; promoter; research study; response; small molecule; synuclein; transcription factor

DESCRIPTION (provided by applicant): The long-term goals of this project are to understand the molecular mechanisms that underlie the degeneration and death of neurons in Parkinson's Disease (PD) and to use this information in turn to devise treatments to suppress the progression of this disorder in patients. The proposed studies will test the over-arching hypothesis that neuron degeneration and death in PD are due to inactivation of the key enzyme Akt which is required for nerve cell function and survival, and that this is mediated by induction of the stress-responsive protein Trib3, an inhibitor of Akt activation. The specific aims will assess four hypotheses: (1) that Trib3 expression is elevated in PD models and in PD; (2) that Trib3 mediates neuron degeneration and death in models of PD; (3) that Trib3 promotes neuron death by interfering with phosphorylation/activation of Akt; and (4) that induction of Trib3 in PD models and PD is mediated by the transcription factor ATF4. Multiple experimental approaches will be employed. These will include assessing Trib3 expression in toxin- and alpha- synuclein based cell culture models of PD, in animal models of the disease and in post-mortem brain tissues from PD patients and controls; determining whether loss of Trib3 expression, either via shRNAs or gene deletion, protects neurons from death in cell culture and animal models of PD; ascertaining (by over- expression or loss-of function studies) whether Trib3 is responsible for mediating the loss of Akt activity that occurs in cellular models of PD; and establishing whether manipulating expression of ATF4 in culture and animal models influences Trib3 induction in culture and animal models of PD. If successful, these studies will establish Trib3 as a required element in the mechanism that leads to neuron death and degeneration in PD and will provide insight as to how it does this, and how it is induced in this disorder. Such information will be exploited in the long term to formulate new approaches to suppress progression of PD in patients. PUBLIC HEALTH RELEVANCE: Parkinson's disease (PD) is characterized by progressive degeneration and death of specific types of nerve cells. Current treatments are aimed at ameliorating the symptoms of this disease rather than its progression. The proposed studies seek to uncover the molecular mechanisms underlying nerve cell degeneration and death in PD so as to lead to generation of new strategies to suppress disease progression.

描述(申请人提供)：该项目的长期目标是了解帕金森氏病(PD)神经元退化和死亡的分子机制，并利用这些信息反过来设计出抑制患者这种疾病进展的治疗方法。拟议的研究将检验帕金森病神经元变性和死亡是由于神经细胞功能和生存所需的关键酶Akt失活所致，这是通过诱导应激反应蛋白Trib3介导的，Trib3是Akt激活的抑制剂。具体目标将评估四个假设：(1)Trib3在PD模型和PD中的表达升高；(2)Trib3介导PD模型中神经元的退化和死亡；(3)Trib3通过干扰Akt的磷酸化/激活促进神经元死亡；以及(4)Trib3在PD模型和PD中的诱导是由转录因子ATF4介导的。将采用多种实验方法。这些工作将包括评估Trib3在以毒素和α-突触核蛋白为基础的细胞培养模型、疾病的动物模型和PD患者和对照的死后脑组织中的表达；确定Trib3的表达缺失，无论是通过shRNA还是基因缺失，保护细胞培养和PD动物模型中的神经元免于死亡；(通过过度表达或功能丧失研究)确定Trib3是否负责调节发生在PD细胞模型中的Akt活性的丧失；以及确定在培养物和动物模型中操纵ATF4的表达是否会影响PD细胞培养和动物模型中的Trib3的诱导。如果成功，这些研究将建立Trib3作为导致帕金森病神经元死亡和变性的机制中的一个必需元素，并将提供关于它如何做到这一点，以及它是如何在这种疾病中诱导的洞察力。这些信息将被长期利用来制定新的方法来抑制患者帕金森病的进展。 公共卫生相关性：帕金森氏病(PD)的特征是特定类型的神经细胞进行性退化和死亡。目前的治疗目的是改善这种疾病的症状，而不是其进展。这些研究试图揭示帕金森病神经细胞退化和死亡的分子机制，从而产生抑制疾病进展的新策略。