FORE-SCI TRAINING PROGRAM (RFP 08-01)

这项建议的主要目标是提供脊髓损伤(SCI)研究技术的年度培训计划。该计划的参与者将接受深入的动手实验室培训，内容包括：胸椎和颈椎椎板切除手术，大鼠和小鼠的脊柱挫伤和横断损伤，脊柱挫伤设备的操作，地面运动分析(BBB和BMS)，先进的行为技术，脊髓损伤大鼠和小鼠的护理，脊髓组织的组织学准备和显微分析，电生理读数(包括肌电图、MEP和SEP)，椎管内药物和/或细胞的输送方法，包括显微注射和鞘内导管输送。该计划还将强调SCI的人性方面，包括一位SCI委员会认证神经学家的讲座，以及与SCI患者的几次互动讨论，在这些讨论中，他们将讨论日常生活中面临的问题。我们还将通过讲座和讨论，就SCI研究的历史背景、实验设计、数据分析和解释、轨迹追踪等主题提供相关信息。所有受训人员将收到广泛的课程材料，包括一本图文并茂的课程手册和几张包含运动分析相关信息的DVD。该计划的目标是，所有参与者在完成计划后都将熟练地进行SCI研究，并能够将在OSU培训计划中学到的技术传授给他们国内实验室的其他成员。

The major objective of this proposal is to provide an anual training program in spinal cord injury (SCI) research techniques. Participants in this program will receive in depth hands-on laboratory training in: Thoracic and cervical laminectomy surgeries, spinal contusion and transection injuries in rats and mice, operating spinal contusion injury devices, overground locomotor analysis (BBB and BMS), advanced behavioral techniques, care of SCI rats and mice, histological preparation and microscopic analysis of spinal tissue, electrophysiological readouts (including EMGs, MEPs, and SEPs), methods for intraspinal delivery of drugs and/or cells including microinjections and intrathecal catheter delivery. This program will also emphasize the human aspect of SCI by including a lecture from a SCI board certified neurologist and several interactive discussions with individuals with SCI in which the issues they face in daily life will be discussed. We also will provide relevant information via lectures and discussions on such topics as the historical background on SCI research, experimental design, data analysis and interpretation, tract tracing as well as other topics. All trainees will receive extensive course materials including an illustrated course manual and several DVDs containing locomotor analysis related information. It is the goal of the program that all participants will be proficient in performing SCI research after completion of the program and will be able to teach the techniques learned in the OSU training program to other members of their home laboratory.