Paramagnetic Microbead-based Surface Plasmon Microarray Detection of Toxins and T

基于顺磁微珠的表面等离子体芯片检测毒素和 T

• 批准号: 8433156
• 负责人: Ernest Fitch Guignon
• 金额: $ 25万
• 依托单位: CIENCIA, INC.
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8433156
• 关键词: Animals; Antibodies; Automation; Bacterial Toxins; Biological; Blood; Clinical; Computer software; Coupled; Detection; Development; Environment; Fluorescence; Gold; Hour; Human; Laboratories; Ligands; Liquid substance; Measurement; Microspheres; Monitor; Patients; Phase; Population; Preparation; Protocols documentation; Reagent; Saliva; Sampling; Sensitivity and Specificity; Site; Surface; Surface Plasmon Resonance; Systems Analysis; Techniques; Technology; Toxin; Urine; Work; aptamer; base; design; instrument; instrumentation; magnetic field; meetings; point of care; prototype; sensor; superparamagnetic beads; toxicant

DESCRIPTION (provided by applicant): The proposed work will result in a characterized prototype instrument that will facilitate the identification of a broad range of analytes in small volumes of human blood, urine, saliva and other readily obtainable biological fluids with little sample preparation. The proposed instrument will incorporate two related analytical approaches, grating-coupled surface Plasmon resonance analysis and surface plasmon-enhanced fluorescence to provide a broad dynamic range coupled with highly sensitive measurements. Analyte selectivity is achieved through the use of capture ligands e.g. antibodies, aptamers, etc. Sample volumes required for the proposed prototype are on the order of 0.3 to 0.5 ml. The proposed instrument will be designed to perform multiple (approx.1000) analyses simultaneously. Gold-clad superparamagnetic nanobeads will be used to increase the selectivity of measurements by transporting analyte to capture sites in a controlled magnetic field. The instrument will be characterized through the use of two commonly encountered toxicants and a bacterial toxin. Software-controlled automation will make the instrument easy to use and suitable for use out of the laboratory. The instrument will provide an inexpensive and efficient way to directly identify or monitor suspected toxicants. PUBLIC HEALTH RELEVANCE: There is a critical need for highly sensitive and specific techniques capable of defining the exposure levels of human and animal populations to the broad range of toxins and toxicants found in the environment. We propose to develop an instrument that will meet this need without many of the limitations that constrain existing technologies. The proposed approach will perform large number (up to a thousand) simultaneous analyses in less than hour. The analyses will identify and quantitate the presence or absence of hundreds of potential toxicants that a patient might encounter without foreknowledge of the contaminant's identity. The technology upon which the proposed instrumentation is based is suitable for use in a clinical laboratory and, with further development extendable to a point-of-care instrument. The instrument will require small sample volumes of biological fluids such as blood, urine or saliva.

描述（由申请人提供）：拟议的工作将产生一种表征的原型仪器，该仪器将有助于在少量人体血液、尿液、唾液和其他易于获得的生物液体中识别广泛的分析物，而样品制备很少。拟议的仪器将结合两个相关的分析方法，光栅耦合表面等离子体共振分析和表面等离子体增强荧光，以提供一个广泛的动态范围，加上高灵敏度的测量。分析物的选择性是通过使用捕获配体，如抗体，适体等，所提出的原型所需的样品体积是在0.3至0.5毫升的顺序。申报仪器将设计为同时进行多项（约1000项）分析。金包覆的超顺磁性纳米珠将用于通过在受控磁场中将分析物运输到捕获位点来增加测量的选择性。将通过使用两种常见毒物和一种细菌毒素对该仪器进行表征。软件控制的自动化将使仪器易于使用，适合在实验室外使用。该仪器将提供一种廉价和有效的方式来直接识别或监测可疑的有毒物质。 公共卫生关系：目前迫切需要有高度敏感和具体的技术，能够确定人类和动物群体接触环境中发现的各种毒素和毒物的程度。我们建议开发一种能够满足这一需求的工具，而不受制约现有技术的许多限制。所提出的方法将在不到一个小时的时间内进行大量（多达一千）的同时分析。分析将确定和定量的存在或不存在的数百个潜在的有毒物质，病人可能会遇到没有预知的污染物的身份。所提出的仪器所基于的技术适合在临床实验室中使用，并且进一步开发可扩展到即时护理仪器。该仪器将需要少量的生物流体样品，如血液、尿液或唾液。