Paramagnetic Microbead-based Surface Plasmon Microarray Detection of Toxins and T

描述(由申请人提供)：拟议的工作将导致一种特色化的原型仪器，该仪器将有助于在少量人类血液、尿液、唾液和其他容易获得的生物液体中识别各种分析物，而只需很少的样品制备。该仪器将结合两种相关的分析方法，即光栅耦合表面等离子体激元共振分析和表面等离子体激元增强荧光分析，以提供宽的动态范围和高灵敏度的测量。分析物的选择性是通过使用捕获配体实现的，例如抗体、适配子等。建议的原型所需的样本量约为0.3至0.5毫升。建议的仪器将设计为同时进行多个(约1000个)分析。包金的超顺磁性纳米棒将被用来增加测量的选择性，方法是将分析物输送到受控磁场中的捕获点。该仪器将通过使用两种常见的毒物和一种细菌毒素来确定其特征。软件控制的自动化将使该仪器易于使用，并适合在实验室外使用。该仪器将提供一种廉价而有效的方式来直接识别或监测可疑毒物。 公共卫生相关性：迫切需要高度敏感和具体的技术，能够确定人类和动物群体对环境中发现的各种毒素和毒物的暴露水平。我们建议开发一种工具，它将满足这一需求，而不会受到制约现有技术的许多限制。所提出的方法将在不到一小时的时间内执行大量(多达1000个)同时分析。这些分析将识别和量化患者在事先不知道污染物身份的情况下可能遇到的数百种潜在毒物的存在或不存在。建议的仪器所基于的技术适合在临床实验室中使用，并随着进一步的开发可扩展到护理点式仪器。该仪器将需要少量的生物液体样本，如血液、尿液或唾液。

DESCRIPTION (provided by applicant): The proposed work will result in a characterized prototype instrument that will facilitate the identification of a broad range of analytes in small volumes of human blood, urine, saliva and other readily obtainable biological fluids with little sample preparation. The proposed instrument will incorporate two related analytical approaches, grating-coupled surface Plasmon resonance analysis and surface plasmon-enhanced fluorescence to provide a broad dynamic range coupled with highly sensitive measurements. Analyte selectivity is achieved through the use of capture ligands e.g. antibodies, aptamers, etc. Sample volumes required for the proposed prototype are on the order of 0.3 to 0.5 ml. The proposed instrument will be designed to perform multiple (approx.1000) analyses simultaneously. Gold-clad superparamagnetic nanobeads will be used to increase the selectivity of measurements by transporting analyte to capture sites in a controlled magnetic field. The instrument will be characterized through the use of two commonly encountered toxicants and a bacterial toxin. Software-controlled automation will make the instrument easy to use and suitable for use out of the laboratory. The instrument will provide an inexpensive and efficient way to directly identify or monitor suspected toxicants. PUBLIC HEALTH RELEVANCE: There is a critical need for highly sensitive and specific techniques capable of defining the exposure levels of human and animal populations to the broad range of toxins and toxicants found in the environment. We propose to develop an instrument that will meet this need without many of the limitations that constrain existing technologies. The proposed approach will perform large number (up to a thousand) simultaneous analyses in less than hour. The analyses will identify and quantitate the presence or absence of hundreds of potential toxicants that a patient might encounter without foreknowledge of the contaminant's identity. The technology upon which the proposed instrumentation is based is suitable for use in a clinical laboratory and, with further development extendable to a point-of-care instrument. The instrument will require small sample volumes of biological fluids such as blood, urine or saliva.