Pooled shRNA screens for target discoveries in cancer

混合 shRNA 筛选癌症靶点发现

• 批准号: 8553186
• 负责人: Ji Luo
• 金额: $ 13.47万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8553186
• 关键词: Cancer cell line; Dependency; Genes; Genetic; Goals; Human; Intramural Research Program; Libraries; Malignant Neoplasms; Methods; Normal Cell; Publishing; Research Personnel; Resources; Technology; cancer cell; cost effective; small hairpin RNA; tool

BACKGROUNDRNAi has become a powerful genetic tool for dissecting genetic dependencies in cancer cells. We have developed methods and technologies to conduct rapid and cost-effective shRNA library screens to identify genetic dependencies in cancer cells.PURPOSEIn this project we will collaborate with other investigators (primarily in the NCI Intramural Program) to enable them to conduct shRNA screens in their cancer cell lines.SIGNIFICANT MATERIALS AND METHODSPooled shRNA library targeting most annotated human genes.FY2012 ACCOMPLISHMENTWe have initiated a number of shRNA screens in difference cancer cell lines and one such screen has been published already.

GROUNDRNAi已成为剖析癌细胞遗传依赖性的强大遗传工具。我们已经开发了方法和技术来进行快速和具有成本效益的shRNA文库筛选，以确定癌细胞中的遗传依赖性。（主要是在NCI校内计划）重要材料和方法靶向大多数注释的人类基因的混合shRNA文库。FY2012完成我们已经启动了一些在不同的癌细胞系中进行shRNA筛选，并且已经发表了一个这样的筛选。