Electrophysiology

电生理学

• 批准号: 8243536
• 负责人: Arrigoni Elda
• 金额: $ 17.8万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2015-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8243536
• 关键词: Affect; Agonist; Animals; Brain; Cell Nucleus; Cells; Core Facility; Data; Data Analyses; Dynorphins; Electrophysiology (science); Ensure; Equipment; Fluorescence Microscopy; Goals; Graph; Histology; Image; Image Analysis; Imagery; Immunohistochemistry; In Vitro; Individual; Label; Laboratories; Methods; Monitor; Neurons; Neurotransmitters; Operative Surgical Procedures; Peptide Receptor; Pharmaceutical Preparations; Preparation; Process; Prosencephalon; Publications; Research Personnel; Resource Sharing; Resources; Sampling; Services; Sleep; Sleep Apnea Syndromes; Slice; Solutions; Synapses; Techniques; Update; basal forebrain; channel blockers; computerized data processing; fluorescence microscope; hypocretin; in vivo; meetings; patch clamp; prevent; research study; response

The Electrophysiology Core will provide centralized services for electrophysiological studies, including preparation of in vitro brain slices, patch-clamp recordings, immunohistochemistry, and the associated image and data analysis. Project 3 (Scammell) and Project 5 (Chamberlin) will use this Core facility. Project 3 will use the services of the Core to examine how orexin and dynorphin increase forebrain activity. Specifically, these experiments will define the pre- and post-synaptic effects and the mechanism through which these neurotransmitters affect cortically-projecting neurons in the basal forebrain. We will also determine the peptide receptors and conductances involved in these responses. Project 5 will use the Electrophysiology Core to determine whether neurons of the Kolliker-Fuse (KF) nucleus are chemosensitive. We will determine which KF neurons are intrinsically responsive to changes of pH, and we will identify the mechanisms underlying these responses. These Projects will benefit from the expertise and many shared resources of the Core.

电生理学核心将为电生理学研究提供集中服务，包括体外脑切片的制备、膜片钳记录、免疫组织化学以及相关的图像和数据分析。项目 3 (Scammell) 和项目 5 (Chamberlin) 将使用此核心设施。项目 3 将使用核心的服务来检查食欲素和强啡肽如何增加前脑活动。具体来说，这些实验将定义突触前和突触后效应以及这些神经递质影响基底前脑皮质投射神经元的机制。我们还将确定参与这些反应的肽受体和电导。项目 5 将使用电生理学核心来确定 Kolliker-Fuse (KF) 核的神经元是否对化学敏感。我们将确定哪些 KF 神经元本质上对 pH 值的变化有反应，并且我们将确定这些反应背后的机制。这些项目将受益于专家的专业知识和许多共享资源 核。