Proteomics Core

蛋白质组学核心就以下七个实验与这四个项目进行或咨询。所涉及的主要技术有2D-DGE、质谱学和OFFGEL分馏。 我们将提供的主要实验包括： (1)用2D-DGE分析TNT、TnL、RLC、TM、MyBP-C、CAPZ和FAK的磷酸化水平。 (2)用2-D-DGE研究亚蛋白质组肌原纤维蛋白表达谱(亚组异型转换水平随丰度的变化)。 (3)用凝胶内消化或从溶液中消化的OFFGEL组分中鉴定蛋白质，然后在高质量准确度/分辨率质谱仪上进行LC-MS/MS分析。 (4)用LC-MS/MS鉴定目的蛋白的磷酸化位点。 (5)利用Oxy印迹、双向凝胶、质谱学等方法鉴定氧化蛋白。 (6)用MALDI-TOF/TOF和LC-MS/MS鉴定交联点。 (7)确定心肌中脂质的变化和/或丰度。

The Proteomics Core conducts or consults with the four projects on the seven following experiments. The major techniques involved are 2D-DIGE, mass spectrometry, and OFFGEL fractionation. The major experiments we will provide are: (1) To analyze TnT, Tnl, RLC,TM, MyBP-C, CapZ, and FAK phosphorylation levels using 2D-DIGE. (2) To investigate the sub-proteomic myofibrillar protein expression profile (isoform switch levels along with abundance changes) with 2-D-DIGE. (3) To identify proteins from polyacrylamide gels by in-gel digest or from OFFGEL fractions digested in solution followed by LC-MS/MS analysis on a high mass accuracy/resolution mass analyzer. (4) To identify the phosphorylation sites of proteins of interest by LC-MS/MS. (5) To identify oxidized proteins by Oxyblot, 2D-diagonal gels, mass spectrometry. (6) To identify the cross-linked sites by MALDI-TOF/TOF and LC-MS/MS. (7) To identify alterations and/or abundance of lipids in the myocardium.