Proteomics Core

蛋白质组学核心

• 批准号: 8380022
• 负责人: Chad Michael Warren
• 金额: $ 29.37万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8380022
• 关键词: Cardiac; Consult; Fractionation; Gel; Lipids; Mass Spectrum Analysis; Molecular Profiling; Myocardium; PTK2 gene; Phosphorylation; Phosphorylation Site; Protein Isoforms; Proteins; Proteomics; Resolution; Site; Solutions; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Techniques; crosslink; interest; mass analyzer; polyacrylamide gels; protein expression; research study

The Proteomics Core conducts or consults with the four projects on the seven following experiments. The major techniques involved are 2D-DIGE, mass spectrometry, and OFFGEL fractionation. The major experiments we will provide are: (1) To analyze TnT, Tnl, RLC,TM, MyBP-C, CapZ, and FAK phosphorylation levels using 2D-DIGE. (2) To investigate the sub-proteomic myofibrillar protein expression profile (isoform switch levels along with abundance changes) with 2-D-DIGE. (3) To identify proteins from polyacrylamide gels by in-gel digest or from OFFGEL fractions digested in solution followed by LC-MS/MS analysis on a high mass accuracy/resolution mass analyzer. (4) To identify the phosphorylation sites of proteins of interest by LC-MS/MS. (5) To identify oxidized proteins by Oxyblot, 2D-diagonal gels, mass spectrometry. (6) To identify the cross-linked sites by MALDI-TOF/TOF and LC-MS/MS. (7) To identify alterations and/or abundance of lipids in the myocardium.

蛋白质组学核心在以下七个实验中指导或咨询这四个项目。所涉及的主要技术是2D-DIGE，质谱法和OFFGEL分馏。