Stem Cell Core

核心A：虽然多能干细胞及其分化的后代对心脏有巨大的希望 维修，他们的日常文化要求很高，需要相当的专业知识和持续的关注 质量控制。根据我们之前对这些细胞的丰富经验，我们发现集中化 细胞培养努力是确保大规模生产的一致性和效率的最佳方式 未分化和已分化的细胞。因此，干细胞核心的主要目标将是产生 大量未分化的胚胎干细胞(ESCs)，诱导的多能干细胞(IPSCs)， 骨髓间充质干细胞(MSCs)和心脏衍生品，用于分配给本项目中的各个项目 程序。核心还将与项目2中的调查人员密切合作，以优化 犬胚胎干细胞来源的心肌细胞。最后，核心还将开发和共享针对 ESCs和IPSCs的基因修饰，包括通过 细菌人工染色体中的转基因。总而言之，核心将承担三个具体目标： 1)向各个项目提供完成其任务所需的干细胞和心脏衍生品 各自的目标，2)发展BAC转基因和优化经修饰的BAC进入人体 ESCs和IPSCs)，以及3)提供有关体外实验的培训和技术援助，包括 多能干细胞或其分化的后代。

Core A: While pluripotent stem cells and their differentiated progeny have tremendous promise for cardiac repair, their routine culture is demanding and requires considerable expertise and continuous attention to quality control. Based on our extensive prior experience with these cells, we have found that centralization of cell culture efforts is the best way to ensure consistency and efficiency in the large-scale production of undifferentiated and differentiated cells. Hence, the primary goal ofthe Stem Cell Core will be to generate large numbers of undifferentiated embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), mesenchymal stem cells (MSCs) and cardiac derivatives for distribution to the individual projects in this program. The Core will also work closely with investigators in Project 2 to optimize the derivation of cardiomyocytes from canine ESCs. Finally, the Core will also develop and share optimized protocols for the genetic modification of ESCs and iPSCs, including the generation of cell type specific reporter lines via transgenesis in bacterial artficial chromosomes (BACs). In sum, the Core will undertake three specific aims: 1) to provide the individual projects with the stem cells and cardiac derivatives needed to complete their respective aims, 2) to develop BAC transgenesis and optimize the delivery of modified BACs into human ESCs and iPSCs), and 3) to provide training and technical assistance with in vitro experiments involving pluripotent stem cells or their differentiated progeny.