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Analytical and Preparative Core

分析和准备核心

基本信息

批准号：
8238302
负责人：
FREDERICK C. DE BEER
金额：
$ 21.59万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8238302
关键词：
2-Mercaptoethanol Acrylamides Acute Acute-Phase Reaction Adenovirus Vector Adenoviruses Aliquot Ampholyte Mixtures Animals Antibodies Apolipoproteins Apolipoproteins A Argon Autoradiography Binding Biological Assay Blood Bolus Infusion Buffers CETP gene COS-7 Cell Canada Cardiovascular system Cells Cellulose Centrifugation Chemicals Cholesterol Chromatography Collaborations Collection Coupled Culture Media Cultured Cells Cyanogen Bromide Cytolysis Density Gradient Centrifugation Diagnostic Drug or chemical Tissue Distribution Dyes Edetic Acid Exclusion Fat emulsion Fluorescence Fluorescent Dyes Fractionation Freeze Drying Freezing Gases Gel Gel Chromatography Generations Genes Glass Hand Helper Viruses High Density Lipoproteins Human Human Volunteers Imagery In Vitro Incubated Individual Inflammation Injection of therapeutic agent Instruction Iodination reaction Iodine Ion-Exchange Chromatography Procedure Isoelectric Focusing Kentucky Label Ligands Lipids Lipoproteins London Low-Density Lipoproteins Lysophospholipids Manufacturer Name Mediating Metabolism Methods Methylene Chloride Modification Mus Natural regeneration Nitrogen Nonesterified Fatty Acids Phase Phospholipase Phospholipids Plant Resins Plaque Assay Plasma Preparation Procedures Process Production Protein Isoforms Proteins Pyroxylin Quality Control Radioactivity Radioisotopes Radiolabeled Reaction Recombinant Proteins Recombinants Recovery Research Resources Sampling Sepharose Serum Services Solid Speed Standardization Sterility Structure of jugular vein Superose 12 Tail Techniques Temperature Thick Time Triglycerides Tube Tyramine Ultracentrifugation Universities Urea Veins Very low density lipoprotein Vial device Viral Virion Virus Western Blotting cholesteryl oleyl ether citrate carrier defective adenoviral vector density evaporation experience fast protein liquid chromatography gel electrophoresis helper-dependent adenoviral vector immunoaffinity chromatography in vivo interest iron oxide mouse model particle polyacrylamide gels pressure programs radiotracer reconstitution research study scale up sodium sulfate uptake vector

项目摘要

The purpose of Core B is to facilitate research and stimulate collaboration between the individual projects. Specifically, Core B will offer the following services: 1. Lipoprotein isolation and characterization 2. Plasma lipoprotein analysis 3. Plasma lipid analysis 4. Labeling of proteins/lipoproteins with radioisotopes and fluorescent dyes 5. HDL remnant particle purification and characterization 6. Production of replication-defective adenoviral vectors and helper-dependent adenoviral vectors 7. Purification of total SAA and SAA isoforms 8. Isoelectric focusing Centralization of these facilities will permit an efficient use of resources for the Program and experienced technical staff will provide optimal quality control and standardization across Projects.

核心B的目的是促进研究和促进各个项目之间的合作。具体而言，Core B将提供以下服务： 1.脂蛋白的分离和鉴定 2.血浆脂蛋白分析 3.血脂分析 4.用放射性同位素和荧光染料标记蛋白质/脂蛋白 5.高密度脂蛋白残留粒子的提纯与表征 6.复制缺陷型腺病毒载体和辅助子依赖型腺病毒载体的制备 7.总SAA和SAA异构体的纯化 8.等电聚焦这些设施的集中化将允许有效地使用计划和经验丰富的资源技术人员将为项目提供最佳的质量控制和标准化。