Dust and IgE Analysis Core

灰尘和 IgE 分析核心

• 批准号: 8392356
• 负责人: Dennis R Ownby
• 金额: $ 9.25万
• 依托单位: HENRY FORD HEALTH SYSTEM
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-06 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8392356
• 关键词: Aliquot; Allergens; American; Antibodies; Archives; Area; Bar Codes; Biological Assay; Budgets; Childhood; Clinical; Computer software; Containment; Core Facility; Data; Data Analyses; Databases; Dust; Endotoxins; Enzyme-Linked Immunosorbent Assay; Equilibrium; Equipment; Extrinsic asthma; Funding; Genetic Transcription; Health Sciences; House Dust; Hypersensitivity; IgE; Immune; Immunoassay; Immunology; Infant; Instruction; Laboratories; Measurement; Measures; Minor; Pathologist; Patient Care; Performance; Personal Computers; Plasma; Publications; Quality Control; Reader; Reagent; Reporting; Research Personnel; Resources; Risk; Sampling; Serum; Shipping; Ships; System; Test Result; Testing; Time; Universities; Variant; base; biobank; college; cost; data exchange; data integration; discount; expectation; instrument; microbiome; pediatric department; pet animal; programs; quality assurance; square foot

PROJECT SUMMARY (See instructions): The purpose of Core E is to provide high quality results for total and allergen-specific IgE to Projects 2 and 4. The value of centralizing this testing is to reduce variation in the results and to gain purchasing efficiency by combining ordering for all reagents to a single laboratory. The Pediatric Allergy-Immunology Laboratory at Georgia Health Sciences University is CLlA-certified for patient care assays and participates in the quality , assurance program of the College of American Pathologists (CAP) for IgE testing. This laboratory has performed all of the IgE tests for the WHEALS study to date. The coefficients of variation for repeat assays are consistenly in the range of 6% or less. Data from all assays is transmitted directly electronically from the analytic instruments to the study coordinators in Detroit for entry into the study database. This eliminates the risks of data corruption from transcription and transmission of data. Dr. Dennis Ownby, who will oversee the performance of the IgE assays, is highly knowledgeable in the area of IgE testing and he will assure the quality of the results reported for analysis in the proposed studies. A system of careful sample tracking and identification based on bar coding all samples has been developed which minimizes the risks of sample loss during shipping or of not matching samples and the results from testing the sample. As in the past, 1% of all samples will be re-assayed weeks to months later to assure quality control and to assure that all samples are correctly identified.

项目总结(见说明)： 核心E的目的是为项目2和4提供高质量的总IgE和过敏原特异性IgE结果。 集中这项测试的价值是通过将所有试剂的订购合并到一个实验室来减少结果的差异并提高采购效率。佐治亚州健康科学大学的儿科过敏-免疫学实验室在患者护理检测方面获得了CLLA认证，并参与了美国病理学家学会(CAP)的IgE测试质量保证计划。到目前为止，该实验室已经为WHEALS研究进行了所有的IgE测试。重复测定的变异系数一致在6%或更小的范围内。所有化验的数据都直接从分析仪器以电子方式传输到底特律的研究协调员，以便输入研究数据库。这消除了数据转录和传输带来的数据损坏风险。Dennis Ownby博士将监督IgE检测的性能，他在IgE检测领域具有高度的知识，他将确保在拟议的研究中报告的分析结果的质量。一种仔细的样本跟踪系统和 已经开发了基于条形码的所有样品的识别，它将样品在运输过程中丢失或样品与测试样品的结果不匹配的风险降至最低。与过去一样，1%的样本将在几周至几个月后重新进行化验，以确保质量控制和确保所有样本都得到正确识别。