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Genome wide siRNA screen for identifying host factors required for ZAP function

全基因组 siRNA 筛选，用于鉴定 ZAP 功能所需的宿主因子

基本信息

批准号：
8289157
负责人：
MARGARET R MACDONALD
金额：
$ 8.48万
依托单位：
ROCKEFELLER UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-03-01 至 2014-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8289157
关键词：
Affect Alphavirus Animals Antiviral Agents Arboviruses Area Arthritis Biological Assay Bioterrorism Boxing Categories Cells Cessation of life Commit Complex Development Disease Disease Outbreaks Ebola virus Encephalitis Exanthema Exhibits Family Fever Filoviridae Frankfurt-Marburg Syndrome Virus Future Genes Goals Human Human Genome Immune response Infection Integration Host Factors Interferons Knowledge Laboratories Lead Letters Mediating National Institute of Allergy and Infectious Disease Natural Immunity Outcome Pathway interactions Patients Process Proteins RNA Helicase Reagent Research Resources Retroviridae Screening procedure Sindbis Virus Small Interfering RNA Specificity Techniques Testing Togaviridae Toxic effect Universities Validation Viral Virus Work Zinc Fingers animal morbidity base cell type cofactor combat genome-wide high throughput screening human morbidity inhibitor/antagonist innovation mortality novel strategies novel therapeutic intervention pathogen prevent protein function prototype success treatment strategy virology

项目摘要

DESCRIPTION (provided by applicant): Viruses in the Alphavirus genus of the Togaviridae family cause significant human and animal morbidity and mortality. There is currently no specific treatment for diseases caused by these viruses. The zinc-finger antiviral protein (ZAP) is a host protein that demonstrates potent inhibition of viruses in this genus, as well as viruses in the Retroviridae and Filoviridae families. The objectives of this project are to use a genome-wide siRNA screening approach to identify host factors that are required for, or facilitate, ZAP function. We anticipate the results will help provide a comprehensive picture of the factors important for ZAP function. This information will provide the basis for interpretation of the curret information regarding ZAP's function and will stimulate new hypothesis-driven research directions. Ultimately, ideas for new approaches to treatment may be stimulated by this work, which may help prevent the devastating diseases caused by these viruses. PUBLIC HEALTH RELEVANCE: Viruses in the Alphavirus genus of the Togaviridae family cause fever, rash, arthritis, encephalitis and death. There are no specific treatments available to combat these diseases. The zinc-finger antiviral protein (ZAP) is a host protein that potently inhibits replication of the alphaviruses. The studies described in this application will screen the entire human genome for those factors that participate in ZAP's inhibitory process. Knowledge gained may lead to new treatment options.

描述(由申请人提供)：Togaviridae家族的甲型病毒属病毒会导致严重的人类和动物发病率和死亡率。目前还没有针对这些病毒引起的疾病的特效药。锌指抗病毒蛋白(ZAP)是一种宿主蛋白，显示出对该属病毒以及逆转录病毒科和丝状病毒科病毒的有效抑制作用。该项目的目标是使用全基因组siRNA筛选方法来确定ZAP功能所需或促进的宿主因子。我们预计结果将有助于全面了解ZAP功能的重要因素。这些信息将为解释有关ZAP功能的最新信息提供基础，并将刺激新的假设驱动的研究方向。最终，这项工作可能会激发治疗新方法的想法，这可能有助于防止这些病毒造成的毁灭性疾病。公共卫生相关性：Togaviridae家族的甲型病毒属病毒会导致发烧、皮疹、关节炎、脑炎和死亡。目前还没有特效的治疗方法。与这些疾病作斗争。锌指抗病毒蛋白(ZAP)是一种宿主蛋白，能有效地抑制甲型病毒的复制。本申请中描述的研究将筛选人类全基因组中那些参与ZAP抑制过程的因子。所获得的知识可能会导致新的治疗选择。