Antibacterial activities of natural minerals and alternative treatment for infect

天然矿物质的抗菌活性和感染的替代治疗

• 批准号: 8250286
• 负责人: SHELLEY E HAYDEL
• 金额: $ 32.89万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8250286
• 关键词: Address; Affect; Africa; Amputation; Anti-Bacterial Agents; Antibiotic Resistance; Antibiotic Therapy; Antibiotics; Antimicrobial susceptibility; Bacteria; Bacterial Infections; Biochemistry; Cells; Characteristics; Child; Chronic; Cicatrix; Clinical; Communicable Diseases; Cutaneous; Deformity; Developing Countries; Development; Disease; Effectiveness; Goals; Growth; Healed; Health; Incidence; Individual; Infection; Infectious Skin Diseases; Ivory Coast; Joints; Legal patent; Lesion; Life; Medical; Microbiology; Minerals; Modality; Molecular Biology; Multi-Drug Resistance; Mus; Mycobacterium ulcerans; Necrosis; Outcome; Painless; Pattern; Predisposition; Property; Recording of previous events; Research; Research Proposals; Skin Nodule; Skin Tissue; Soft Tissue Disorder; Source; Stress; Subcutaneous Tissue; Testing; Therapeutic; Therapeutic Agents; Ulcer; Work; World Health Organization; Wound Healing; alternative treatment; bactericide; base; clay; cost effective; disability; effective therapy; healing; killings; methicillin resistant Staphylococcus aureus; nanomaterials; pathogen; pathogenic bacteria; prevent; research study; resistant strain; topical antibacterial

DESCRIPTION (provided by applicant): Our capacity to properly address the worldwide incidence of infectious diseases lies in the ability to detect, prevent, and effectively treat these infections. With increasing overuse and misuse of antibiotics, infectious bacteria have become increasingly antibiotic-resistant. Therefore, identifying and analyzing inhibitory agents is a worthwhile endeavor since few new antibacterial compounds have been produced in recent decades. The use of natural nanominerals to heal skin infections has been evident since the earliest recorded history, and specific clay minerals may prove valuable in the treatment of bacterial diseases, including infections for which there are no effective antibiotics, such as Buruli ulcer and multi- drug resistant infections. The rationale for the proposed research is based on several observations and realizations: (a) Specific clay minerals have been used to treat and heal children afflicted with Buruli ulcer, a necrotic cutaneous infection caused by Mycobacterium ulcerans; (b) Susceptibility testing of the minerals that were used to treat Buruli ulcer and two additional minerals revealed bactericidal or bacteriostatic activity against several different bacterial pathogens, including antibiotic-resistant strains; and (c) Use of antibacterial minerals would provide an inexpensive, alternative therapeutic treatment for topical infections. Considering the lack of therapeutics for treating necrotic M. ulcerans infections and other life-threatening skin infections, such as MRSA, our goals are to validate the observations that specific minerals can heal bacterial infections by investigating broad-spectrum antibacterial characteristics of the clay minerals and determining the mechanism of action of these bioactive minerals. We propose to (1) assess the broad-spectrum antibacterial effects of natural nanominerals, (2) evaluate the ability of hydrated minerals to treat topical bacterial infections in mice, and (3) determine the mechanism of action of the natural antibacterial minerals and how the minerals structurally and transcriptionally affect bacterial cells. PUBLIC HEALTH RELEVANCE: Through the use of antimicrobial susceptibility testing, we have established that certain clay minerals kill or inhibit the growth of pathogenic bacteria. In an era when few new antibiotics are being discovered, we are investigating natural nanominerals as a potential source of alternative therapeutic agents. Using microbiology, molecular biology, biochemistry, and medical/clinical approaches, we seek to determine the susceptibility patterns of bacterial pathogens to these antibacterial minerals, validate the use of specific minerals for the treatment of topical skin infections, and understand the bactericidal mechanism of action.

描述（由申请人提供）：我们正确解决全球传染病发病率的能力在于能够检测，预防和有效治疗这些感染的能力。随着过度使用和滥用抗生素的滥用，传染性细菌变得越来越抗生素。因此，鉴定和分析抑制剂是值得的努力，因为最近几十年来很少生产新的抗菌化合物。自从记录最早的历史以来，使用天然纳米脂蛋白来治愈皮肤感染，并且特定的粘土矿物质可能在细菌疾病治疗中很有价值，包括没有有效的抗生素的感染，例如Buruli溃疡和多种药物耐药性感染。拟议研究的基本原理是基于几种观察结果和实现：（a）特定的粘土矿物被用来治疗和治愈患有Buruli溃疡的儿童，这是由溃疡性分枝杆菌引起的坏死性皮肤感染； （b）用于治疗布鲁利溃疡的矿物质的易感性测试，另外两种矿物质显示出针对几种不同的细菌病原体的杀菌性或抑菌活性，包括抗生素抗性菌株； （c）使用抗菌矿物质将为局部感染提供廉价的替代治疗方法。考虑到缺乏治疗坏死的溃疡性感染和其他威胁生命的皮肤感染的治疗学，我们的目标是验证特定矿物质可以通过研究粘土矿物的广谱抗体特征来治愈细菌感染的观察结果，并确定这些生物学的机械性销售机构。我们建议（1）评估天然纳米脂蛋白的广谱抗菌作用，（2）评估水合矿物治疗小鼠中局部细菌感染的能力，（3）确定天然抗菌矿物质的作用机理，以及矿物质结构和转录对细菌细胞的影响。公共卫生相关性：通过使用抗菌易感性测试，我们确定某些粘土矿物会杀死或抑制致病细菌的生长。在一个很少有新抗生素的时代，我们正在研究天然纳米脂蛋白作为替代治疗剂的潜在来源。使用微生物学，分子生物学，生物化学和医学/临床方法，我们试图确定细菌病原体对这些抗细菌矿物质的易感模式，验证使用特定矿物质来治疗局部皮肤感染，并了解杀菌作用机制。