A low-cost instrument for nucleic acid isolation.

一种用于核酸分离的低成本仪器。

• 批准号: 8393862
• 负责人: Christopher Gerard Cooney
• 金额: $ 28.08万
• 依托单位: AKONNI BIOSYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-10 至 2013-09-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8393862
• 关键词: Acid Fast Bacillae Staining Method; Address; Aspirate substance; Bacillus (bacterium); Blood; Buffers; Cerebrospinal Fluid; Chemistry; Clinical; Cytolysis; DNA; Detection; Diagnostic; Equipment; Feces; Genus Mycobacterium; Gram-Positive Bacteria; Laboratories; Lead; Life; Mechanics; Methods; Modality; Molecular; Molecular Biology; Nucleic Acids; Organism; Performance; Phase; Preparation; Procedures; Process; Production; Protocols documentation; RNA; RNA purification; Reagent; Recovery; Saliva; Sampling; Sputum; Swab; Technology; Temperature; Testing; Urine; Whole Blood; cost; instrument; preclinical study; prototype

DESCRIPTION (provided by applicant): Sample preparation frequently presents a bottleneck for clinical molecular biology workflows. Whereas there are many readout detection modalities, there are only a handful of automated sample preparation workflow strategies. Existing instruments that are built around these sample preparation strategies and chemistries range in cost from $17-$150k, and yet they still do not include an integrated method of lysing difficult-to-disrupt organisms such as gram-positive bacteria or the acid fast bacilli (i.e., Mycobacterium). To address these limitations, we propose to integrate Akonni's sample preparation technologies into a low-cost automated instrument that can process a variety of sample types such as blood, nasopharyngeal swabs, and nasopharyngeal aspirate with a simple-to-use consumable kit. PUBLIC HEALTH RELEVANCE: Molecular diagnostics, which are tests that analyze the DNA and/or RNA in a sample, are becoming increasingly prevalent among clinical laboratory test methods. An important, yet challenging, procedure, required of these tests, is to remove the DNA/RNA from the sample and wash away the impurities. We seek to develop a low-cost automated instrument to address this challenge

描述（由申请人提供）：样品制备通常是临床分子生物学工作流程的瓶颈。尽管有许多读出检测模式，但只有少数自动化样品制备工作流程策略。围绕这些样品制备策略和化学物质构建的现有仪器的成本范围为17 - 15万美元，但它们仍然不包括裂解难以破坏的生物体（例如革兰氏阳性细菌或抗酸杆菌（即，分枝杆菌）。为了解决这些局限性，我们建议将Akonni的样品制备技术整合到一个低成本的自动化仪器中，该仪器可以使用简单易用的耗材套件处理各种样品类型，如血液、鼻咽拭子和鼻咽抽吸物。 公共卫生关系：分子诊断是分析样本中DNA和/或RNA的测试，在临床实验室测试方法中变得越来越普遍。这些测试所需的一个重要但具有挑战性的程序是从样品中去除DNA/RNA并洗掉杂质。我们寻求开发低成本的自动化仪器来应对这一挑战