NEONATAL TRANSLATIONAL

新生儿转化中心位于纽约州立大学下州医疗中心。主要功能有：a)氧诱导视网膜病变的动物模型；2)视网膜氧化应激细胞培养模型；C)生化分析；D)基因谱；e)免疫组织化学、免疫荧光和成像分析。该核心的目的是促进和促进pi之间的合作研究互动；为初级教师、研究员、住院医师和研究科学家建立最先进的转化研究培训核心设施，重点关注氧诱导视网膜病变和氧化应激；招募基础和临床研究人员进行视网膜病变研究。该核心将提供可访问性，以支持本提案中涵盖的正在进行的和新出现的研究项目，这些项目需要该核心提供的各种方法和分析能力。这个核心也将促进正在开发早产儿视网膜病变新疗法的研究人员之间正在进行的和新的合作。每一项服务都被儿科pi广泛使用，对这些服务的需求很大。许多服务和/或设备的使用将免费提供给调查人员；这包括所有的分子服务；生化服务;设备的使用。为了访问服务，将提供以下服务订购表格。对于方案1，除其他核心外，该核心将负责所有实验阶段，包括动物处理、给药、高氧/缺氧循环、血液和组织采集、样品处理和图像分析。对于方案2，除其他核心外，该核心将负责所有实验阶段，包括细胞电镀，SiRNA干扰，培养基和细胞收获，样品处理和图像分析。对于方案3，新生儿翻译核心将分析所有患者血液样本中的前列腺素、VEGF、sVEGFR-1、IGF-I以及胎盘、脐带和血液样本中VEGF和Notch信号的基因谱。

The Neonatal Translational Core is located in at SUNY Downstate Medical Center. The key functional capabilities are: a) animal models for oxygen-induced retinopathy; 2) cell culture models for oxidative stress in the retina; c) biochemical analyses; d) gene profiling; and e) immunohistochemistry, immunofluorescence and imaging analyses. The aims of this core are to promote and foster collaborative research interactions among PIs; establish state-of-the-art translational research training core facility focusing on oxygen-induced retinopathy and oxidative stress for junior faculty, fellows, residents, and research scientists; and recruit basic and clinical investigators into retinopathy research. Accessibility to this Core will be afforded to support the ongoing and newly emerging research programs covered in this proposal, which requires the kinds of methodologies and analytical capabilities offered by this core. This core will also facilitate ongoing and new collaborations between investigators who are developing novel therapeutic efforts for Retinopathy of Prematurity. Each of these services has been extensively used by the Pediatric PIs and there is great demand for these services. Many of the services and/or use of equipment will be free of charge to investigators; these include all molecular services; biochemical services; use of the equipment. In order to access services the following service order forms will be available. For Protocol 1, this core will be responsible for all experimental phases including animal handling, drug administration, hyperoxia/hypoxia cycling, blood and tissue harvesting, sample processing and image analyses in addition to the other cores. For Protocol 2, this core will be responsible for all experimental phases including plating the cells, SiRNA interference, media and cell harvesting, sample processing and image analyses in addition to the other cores. For Protocol 3, the neonatal translational core will analyze all patient blood samples prostaglandins, VEGF, sVEGFR-1, IGF-I and gene profiling of VEGF and Notch signaling in the placenta, cord and blood samples.