NEONATAL TRANSLATIONAL

新生儿翻译中心位于纽约州立大学下州医疗中心。主要功能包括：a)氧诱导视网膜病变的动物模型；2)视网膜氧化应激的细胞培养模型；c)生化分析；d)基因图谱；e)免疫组织化学、免疫荧光和成像分析。这一核心的目标是促进和促进个人投资机构之间的协作研究互动；为初级教员、研究员、住院医生和研究科学家建立最先进的转化性研究培训核心设施，专注于氧诱导的视网膜病变和氧化应激；并招募基础和临床研究人员参与视网膜病变研究。这一核心的可访问性将用于支持本提案中涵盖的正在进行的和新出现的研究计划，这需要该核心所提供的各种方法和分析能力。这一核心还将促进正在开发早产儿视网膜病变新疗法的研究人员之间正在进行的和新的合作。这些服务每一项都被儿科私人投资机构广泛使用，而且对这些服务的需求很大。许多服务和/或设备的使用将对调查人员免费；这包括所有分子服务；生化服务；设备的使用。为了获得服务，将提供以下服务订单表。对于议定书1，除其他核心外，该核心将负责所有实验阶段，包括动物处理、给药、高氧/低氧循环、血液和组织采集、样品处理和图像分析。对于第二号议定书，除其他核心外，该核心将负责所有实验阶段，包括细胞电镀、siRNA干扰、培养液和细胞采集、样品处理和图像分析。对于协议3，新生儿翻译核心将分析所有患者血液样本中的前列腺素、血管内皮生长因子、sVEGFR-1、IGF-I以及胎盘、脐带和血液样本中的血管内皮生长因子基因图谱和Notch信号。

The Neonatal Translational Core is located in at SUNY Downstate Medical Center. The key functional capabilities are: a) animal models for oxygen-induced retinopathy; 2) cell culture models for oxidative stress in the retina; c) biochemical analyses; d) gene profiling; and e) immunohistochemistry, immunofluorescence and imaging analyses. The aims of this core are to promote and foster collaborative research interactions among PIs; establish state-of-the-art translational research training core facility focusing on oxygen-induced retinopathy and oxidative stress for junior faculty, fellows, residents, and research scientists; and recruit basic and clinical investigators into retinopathy research. Accessibility to this Core will be afforded to support the ongoing and newly emerging research programs covered in this proposal, which requires the kinds of methodologies and analytical capabilities offered by this core. This core will also facilitate ongoing and new collaborations between investigators who are developing novel therapeutic efforts for Retinopathy of Prematurity. Each of these services has been extensively used by the Pediatric PIs and there is great demand for these services. Many of the services and/or use of equipment will be free of charge to investigators; these include all molecular services; biochemical services; use of the equipment. In order to access services the following service order forms will be available. For Protocol 1, this core will be responsible for all experimental phases including animal handling, drug administration, hyperoxia/hypoxia cycling, blood and tissue harvesting, sample processing and image analyses in addition to the other cores. For Protocol 2, this core will be responsible for all experimental phases including plating the cells, SiRNA interference, media and cell harvesting, sample processing and image analyses in addition to the other cores. For Protocol 3, the neonatal translational core will analyze all patient blood samples prostaglandins, VEGF, sVEGFR-1, IGF-I and gene profiling of VEGF and Notch signaling in the placenta, cord and blood samples.