NEONATAL TRANSLATIONAL

新生儿翻译

• 批准号: 8260920
• 负责人: Kay Beharry
• 金额: $ 3.88万
• 依托单位: SUNY DOWNSTATE MEDICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8260920
• 关键词: Animal Model; Animals; Biochemical; Blood; Blood specimen; Cell Culture Techniques; Cells; Charge; Childhood; Clinical Investigator; Clinical Pharmacology; Collaborations; Core Facility; Equipment; Faculty; Fostering; Genes; Harvest; Health Services Accessibility; Human Resources; Hyperoxia; Hypoxia; Image Analysis; Immunofluorescence Immunologic; Immunohistochemistry; Insulin-Like Growth Factor I; Medical center; Methodology; Modeling; Molecular; Neonatal; Oxidative Stress; Oxygen; Patients; Pharmaceutical Preparations; Phase; Placenta; Process; Prostaglandins; Protocols documentation; Recruitment Activity; Research; Research Personnel; Research Training; Retina; Retinal Diseases; Retinopathy of Prematurity; Sampling; Scientist; Services; Signal Transduction; Tissue Harvesting; Training; Translational Research; Umbilical Cord Blood; Vascular Endothelial Growth Factors; design; notch protein; novel therapeutics; programs

The Neonatal Translational Core is located in at SUNY Downstate Medical Center. The key functional capabilities are: a) animal models for oxygen-induced retinopathy; 2) cell culture models for oxidative stress in the retina; c) biochemical analyses; d) gene profiling; and e) immunohistochemistry, immunofluorescence and imaging analyses. The aims of this core are to promote and foster collaborative research interactions among PIs; establish state-of-the-art translational research training core facility focusing on oxygen-induced retinopathy and oxidative stress for junior faculty, fellows, residents, and research scientists; and recruit basic and clinical investigators into retinopathy research. Accessibility to this Core will be afforded to support the ongoing and newly emerging research programs covered in this proposal, which requires the kinds of methodologies and analytical capabilities offered by this core. This core will also facilitate ongoing and new collaborations between investigators who are developing novel therapeutic efforts for Retinopathy of Prematurity. Each of these services has been extensively used by the Pediatric PIs and there is great demand for these services. Many of the services and/or use of equipment will be free of charge to investigators; these include all molecular services; biochemical services; use of the equipment. In order to access services the following service order forms will be available. For Protocol 1, this core will be responsible for all experimental phases including animal handling, drug administration, hyperoxia/hypoxia cycling, blood and tissue harvesting, sample processing and image analyses in addition to the other cores. For Protocol 2, this core will be responsible for all experimental phases including plating the cells, SiRNA interference, media and cell harvesting, sample processing and image analyses in addition to the other cores. For Protocol 3, the neonatal translational core will analyze all patient blood samples prostaglandins, VEGF, sVEGFR-1, IGF-I and gene profiling of VEGF and Notch signaling in the placenta, cord and blood samples.

新生儿翻译中心位于纽约州立大学下州医学中心。主要功能包括：a）氧诱导视网膜病变的动物模型; 2）视网膜氧化应激的细胞培养模型; c）生化分析; d）基因分析; e）免疫组织化学、免疫荧光和成像分析。该核心的目的是促进和促进PI之间的合作研究互动;建立最先进的转化研究培训核心设施，专注于初级教师，研究员，居民和研究科学家的氧诱导视网膜病变和氧化应激;并招募基础和临床研究人员进行视网膜病变研究。将提供该核心的可访问性，以支持本提案中所涵盖的正在进行的和新出现的研究计划，这些计划需要该核心提供的各种方法和分析能力。该核心还将促进正在为早产儿视网膜病变开发新的治疗方法的研究人员之间的持续和新的合作。这些服务中的每一项都被儿科PI广泛使用，并且对这些服务的需求很大。许多服务和/或设备的使用将免费提供给研究者;这些服务包括所有分子服务;生化服务;设备的使用。为了获得服务，将提供以下服务订单。对于方案1，除其他核心外，该核心将负责所有实验阶段，包括动物处理、给药、高氧/缺氧循环、血液和组织采集、样本处理和图像分析。 对于方案2，除其他核心外，该核心将负责所有实验阶段，包括细胞铺板、SiRNA干扰、培养基和细胞收获、样品处理和图像分析。对于方案3，新生儿翻译核心将分析所有患者血液样本中的胎盘素、VEGF、sVEGFR-1、IGF-I以及胎盘、脐带和血液样本中VEGF和Notch信号传导的基因谱。