Flow Cytometry

流式细胞仪

• 批准号: 8302941
• 负责人: JIANKE ZHANG
• 金额: $ 9.46万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2013-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8302941
• 关键词: Apoptosis; Applications Grants; Area; Basic Science; Biochemical; Biological; Biological Sciences; Biology; Cancer Center; Cell Cycle; Cell Cycle Progression; Cell Cycle Stage; Cell Separation; Cells; Characteristics; Cloning; Consult; Cytometry; Data; Data Analyses; Detection; Development; Effector Cell; Floor; Flow Cytometry; Flow Cytometry Shared Resource; Funding; Future; Goals; Immune; Individual; Investigation; Kimmel Cancer Center at the Thomas Jefferson University; Kinetics; Laboratories; Literature; Malignant Neoplasms; Methods; Mission; NCI Center for Cancer Research; Normal Cell; Peer Review; Phenotype; Play; Population; Positioning Attribute; Price; Production; Property; Proteins; Protocols documentation; Publications; Publishing; Reagent; Reproducibility; Research; Research Personnel; Resource Sharing; Resources; Role; Services; Sorting - Cell Movement; Specific qualifier value; Speed; Standardization; Sterility; Structure of thyroid parafollicular cell; Surface; Techniques; Time; Training; Translational Research; Universities; base; biosafety level 3 facility; cancer therapy; cell transformation; cytokine; experimental analysis; fluorescence microscope; genetic manipulation; instrumentation; member; particle; pathogen; programs; single cell analysis; square foot; tumor

The Flow Cytometry Shared Resource provides state-of-the-art cell sorting and analysis capability to the members of the Kimmel Cancer Center at Thomas Jefferson University. This shared resource is located in a dedicated laboratory space of about 600 sq ft on the 6th floor of the Bluemle Life Sciences Building, centrally positioned on the Thomas Jefferson University campus. The Flow Cytometry Shared Resource is equiped with one Cytomation MoFlo cell high-speed sorter, one Coutler EPICS Elite flow cytometer capable of sorting, one Coulter XL-MCL automated analytical cytometer, one FACS Calibur Benchtop Analyzer, one FACScan Benchtop Analyzer, two Celeron 400 workstations, one Nikon fluorescence microscope, a 30 cu ft 4¿C refrigerator, and a 10 cu ft -20¿C freezer. All units are maintained and managed by a dedicated manager/operator. The most commonly used applications are for surface phenotyping and sorting, detection of intracellular cytokines at the single cell level in mixed populations, single cell real time analysis of Ca[2+] mobilization kinetics, studies of apoptosis, cell cycle analysis, and sorting of viable cells in specific stages of the cell cycle. This shared resource allows experimental and data analysis to be performed in a timely manner and with high sensitivity and accuracy. Peer-review, funded cancer center investigators receive priority for the use of this shared resource. Based on increased capabilities for new services, the steady increase in the use of this resource by cancer center members and the recruitment of new investigators, we project that shared resource usage will increase significantly in the near future. In summary, this shared resource plays a critical role in assisting cancer center investigators as they conduct research into the biology and treatment of cancer.

流式细胞术共享资源为Thomas Jefferson大学Kimmel癌症中心的成员提供最先进的细胞分选和分析能力。该共享资源位于位于托马斯杰斐逊大学校园中心的Bluemle生命科学大楼6楼约600平方英尺的专用实验室空间中。流式细胞术共享资源配备一台Cytomation MoFlo细胞高速分选仪，一台Coutler EPICS Elite流式细胞仪