Flow Cytometry

FCSR摘要 Sidney Kimmel癌症中心流式细胞术共享资源(FCSR)提供最先进的 Sidney Kimmel癌症研究人员的荧光激活细胞分选和分析能力 中心和费城地区。最常用的应用是多色电池表面 表型，细胞内细胞因子和信号分子的检测，转基因测量，研究 包括细胞凋亡、细胞周期分析、细胞增殖和实时分析钙动员动力学。这个 分类器、分析器和工作站允许以经济、可靠和准确的方式表征广泛的 各种生物样本。提供的这些服务可以量身定做，以适应各种实验设计 在田野里。FCSR提供关于当前协议、分析技术和数据呈现的咨询。这 资源在帮助SKCC成员执行各种直接相关的研究项目方面发挥着关键作用 对癌症的生物学和治疗的了解。该设施目前的目标包括：1)提供 为分析性细胞仪数据采集和细胞仪细胞分选提供高质量和实惠的服务 各种类型的生物颗粒；2)与SKCC成员协商，确定分析细胞术 需要，帮助开发适当的协议和技术，并提供技术支持 分析，包括使用BD、FAC、DIVA和FlowJo的数据分析和解释和/或培训 软件；3)为SKCC成员提供全天候使用自动分析流式细胞术的培训 用于称职和有效的常规分析的仪器。

FCSR Summary The Sidney Kimmel Cancer Center Flow Cytometry Shared Resource (FCSR) provides state-of-the-art fluorescence activated cell sorting and analysis capabilities to investigators of the Sidney Kimmel Cancer Center and the Philadelphia region. The most commonly used applications are multi-color cell surface phenotyping, detection of intracellular cytokines and signaling molecules, transfection measurements, studies of apoptosis, cell cycle analysis, cell proliferation, and real time analysis of Ca2+ mobilization kinetics. The sorters, analyzers, and workstations allow for affordable, reliable, and accurate characterization of a wide variety of biological samples. These services provided can be tailored to suit experimental designs in a variety of fields. FCSR provides consultation on current protocols, analysis techniques, and data presentation. This resource plays a critical role in assisting SKCC members performing various research projects directly related to the understanding of the biology and treatment of cancer. Current goals of the facility include: 1) Provide high-quality and affordably priced services for analytical cytometry data acquisition and cytometric cell sorting of various types of biological particles; 2) Consult with SKCC members to determine analytical cytometry needs, aid in the development of appropriate protocols and techniques, and provide technical support for analysis including data analysis and interpretation and/or training in using BD FACS DIVA and FlowJo softwares; 3) Provide training to SKCC members for 24/7 use of automated analytical flow cytometry instrumentation for competent and efficient routine analysis.