Neural Substrates of Electroacupuncture in Cardiovascular Control

电针控制心血管的神经基质

• 批准号: 8456056
• 负责人: John C Longhurst
• 金额: $ 36.65万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8456056
• 关键词: Abbreviations; Acupuncture procedure; Acute; Adverse effects; American; Animals; Area; Automobile Driving; Biologic Characteristic; Blood Pressure; Brain; Cardiovascular system; Cell Nucleus; Cholecystokinin A Receptor; Cholecystokinin Receptor; Clinical; Conscious; Data; Disease; Electroacupuncture; Endorphins; Enkephalins; Figs - dietary; Genes; Glutamates; Goals; Grant; Hour; Hypertension; Hypotension; Hypothalamic structure; Incidence; Investigation; Knowledge; Medical; Messenger RNA; Methods; Midbrain structure; Modality; Modeling; Molecular; Morbidity - disease rate; Myocardial Ischemia; National Center for Complementary and Alternative Medicine; National Heart, Lung, and Blood Institute; Neurons; Neurotransmitters; Opioid; Oranges; Pathway interactions; Patients; Physicians; Physiological; Play; Population; Pro-Opiomelanocortin; Process; Production; Rattus; Reflex action; Regulation; Research; Role; Spinal Cord; Standardization; Structure of nucleus infundibularis hypothalami; Tetragastrin; Text; Thoracic spinal cord structure; Treatment Protocols; Visceral; Whole Organism; aged; hemodynamics; hypertension treatment; improved; insight; mRNA Expression; midbrain central gray substance; mortality; neuromechanism; neurophysiology; neuroregulation; paraventricular nucleus; preproenkephalin; raphe nuclei; relating to nervous system; response

DESCRIPTION (provided by applicant): We have investigated the mechanisms by which acupuncture, particularly electroacupuncture (EA) regulates blood pressure (BP). EA has the capability to lower elevated BP and to alleviate demand-induced myocardial ischemia. Its actions are slow in onset but its duration of action is prolonged. Approximately 70% of experimental subjects with acute reflex-induced elevations in BP respond to EA. The most recent grant has focused on a long-loop pathway extending from the hypothalamic arcuate nucleus to the midbrain ventrolateral periaqueductal gray (vlPAG) to the medullary midline nucleus raphe' pallidus (NRP) and then the rostral ventrolateral medulla (rVLM) where the activity of premotor sympathetic neurons is inhibited by EA. We specifically identified a reinforcing reciprocating network between the arcuate and the vlPAG that serves to prolong the action of EA. We also defined an important role for the NRP as a nucleus that processes glutamatergic input from the vlPAG and through a 5-HT1A rVLM mechanism modulates sympathetic outflow. The central hypothesis of this competitive renewal is that a standardized course of acupuncture treatment and established methods convert nonresponders to responders to increase EA's efficacy in reducing elevated blood pressure (BP), particularly through its inhibitory action in the hypothalamic paraventricular nucleus (PVN). The present grant will focus on four new areas: 1) the role of the PVN modulated during EA by opioid (enkephalin and endorphinergic) inputs from the arcuate and through projections to the rVLM and IML inhibits reflex-evoked sympathetic activation, 2) the mechanism through which repetitive EA in conscious cold-induced hypertensive (CI-HTN) rats lowers BP, 3) transcriptional mechanisms regulating enkephalin and endorphin production in the rVLM and arcuate during repetitive stimulation in EA's action to lower BP in CI-HTN rats and 4) cholecystokinin neuromodulation of opioid activity in the PVN of animals unresponsive to the actions of EA. Anatomical, physiological, electrophysiological, pharmacological and molecular approaches will be used in the proposed studies that are highly relevant to patients that have the potential to benefit from clinical acupuncture.

描述（由申请人提供）：我们研究了针灸，特别是电针（EA）调节血压（BP）的机制。电针具有降低血压和减轻心肌缺血的作用。它的作用是缓慢的，但其作用时间是延长。大约70%的实验对象与急性反射引起的血压升高响应EA。最近的资助集中在从下丘脑弓状核延伸到中脑腹外侧导水管周围灰质（vlPAG）到延髓中线中缝苍白核（NRP），然后延伸到延髓头端腹外侧区（rVLM）的长环通路，其中运动前交感神经元的活性被EA抑制。我们特别确定了弓形和vlPAG之间的强化往复网络，其用于延长EA的作用。我们还将NRP定义为处理来自vlPAG的交感神经能输入并通过5-HT 1A rVLM机制调节交感神经流出的核的重要作用。这种竞争性更新的中心假设是，标准化的针灸治疗过程和既定的方法将无反应者转化为反应者，以增加EA降低血压升高（BP）的功效，特别是通过其在下丘脑室旁核（PVN）中的抑制作用。目前的赠款将集中在四个新领域：1）阿片类药物在电针过程中对PVN的调节作用（脑啡肽和内啡肽）从弓状和通过投射到rVLM和IML的输入抑制反射诱发的交感神经激活，2）在清醒的冷诱导的高血压（CI-HTN）大鼠中重复EA降低BP的机制，3）在电针降低CI-HTN大鼠血压的作用中，在重复刺激期间调节rVLM和弓状核中脑啡肽和内啡肽产生的转录机制;胆囊收缩素神经调节阿片活性的动物PVN无反应的EA的行动。解剖学、生理学、电生理学、药理学和分子学方法将用于与有可能从临床针灸中受益的患者高度相关的拟议研究。