Neural Substrates of Electroacupuncture in Cardiovascular Control

电针控制心血管的神经基质

• 批准号: 8456056
• 负责人: John C Longhurst
• 金额: $ 36.65万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8456056
• 关键词: Abbreviations; Acupuncture procedure; Acute; Adverse effects; American; Animals; Area; Automobile Driving; Biologic Characteristic; Blood Pressure; Brain; Cardiovascular system; Cell Nucleus; Cholecystokinin A Receptor; Cholecystokinin Receptor; Clinical; Conscious; Data; Disease; Electroacupuncture; Endorphins; Enkephalins; Figs - dietary; Genes; Glutamates; Goals; Grant; Hour; Hypertension; Hypotension; Hypothalamic structure; Incidence; Investigation; Knowledge; Medical; Messenger RNA; Methods; Midbrain structure; Modality; Modeling; Molecular; Morbidity - disease rate; Myocardial Ischemia; National Center for Complementary and Alternative Medicine; National Heart, Lung, and Blood Institute; Neurons; Neurotransmitters; Opioid; Oranges; Pathway interactions; Patients; Physicians; Physiological; Play; Population; Pro-Opiomelanocortin; Process; Production; Rattus; Reflex action; Regulation; Research; Role; Spinal Cord; Standardization; Structure of nucleus infundibularis hypothalami; Tetragastrin; Text; Thoracic spinal cord structure; Treatment Protocols; Visceral; Whole Organism; aged; hemodynamics; hypertension treatment; improved; insight; mRNA Expression; midbrain central gray substance; mortality; neuromechanism; neurophysiology; neuroregulation; paraventricular nucleus; preproenkephalin; raphe nuclei; relating to nervous system; response

DESCRIPTION (provided by applicant): We have investigated the mechanisms by which acupuncture, particularly electroacupuncture (EA) regulates blood pressure (BP). EA has the capability to lower elevated BP and to alleviate demand-induced myocardial ischemia. Its actions are slow in onset but its duration of action is prolonged. Approximately 70% of experimental subjects with acute reflex-induced elevations in BP respond to EA. The most recent grant has focused on a long-loop pathway extending from the hypothalamic arcuate nucleus to the midbrain ventrolateral periaqueductal gray (vlPAG) to the medullary midline nucleus raphe' pallidus (NRP) and then the rostral ventrolateral medulla (rVLM) where the activity of premotor sympathetic neurons is inhibited by EA. We specifically identified a reinforcing reciprocating network between the arcuate and the vlPAG that serves to prolong the action of EA. We also defined an important role for the NRP as a nucleus that processes glutamatergic input from the vlPAG and through a 5-HT1A rVLM mechanism modulates sympathetic outflow. The central hypothesis of this competitive renewal is that a standardized course of acupuncture treatment and established methods convert nonresponders to responders to increase EA's efficacy in reducing elevated blood pressure (BP), particularly through its inhibitory action in the hypothalamic paraventricular nucleus (PVN). The present grant will focus on four new areas: 1) the role of the PVN modulated during EA by opioid (enkephalin and endorphinergic) inputs from the arcuate and through projections to the rVLM and IML inhibits reflex-evoked sympathetic activation, 2) the mechanism through which repetitive EA in conscious cold-induced hypertensive (CI-HTN) rats lowers BP, 3) transcriptional mechanisms regulating enkephalin and endorphin production in the rVLM and arcuate during repetitive stimulation in EA's action to lower BP in CI-HTN rats and 4) cholecystokinin neuromodulation of opioid activity in the PVN of animals unresponsive to the actions of EA. Anatomical, physiological, electrophysiological, pharmacological and molecular approaches will be used in the proposed studies that are highly relevant to patients that have the potential to benefit from clinical acupuncture.

描述（由申请人提供）：我们已经研究了针灸，特别是电针（EA）调节血压（BP）的机制。EA具有降低血压升高和缓解需求性心肌缺血的能力。其作用开始缓慢，但持续时间延长。大约70%的急性反射性血压升高的实验对象对EA有反应。最近的资助集中在从下丘脑弓状核到中脑腹外侧导水管周围灰质（vlPAG），再到中脑中线中脑白质核（NRP），然后是延髓吻侧腹外侧核（rVLM）的长环通路上，在这里，运动前交感神经元的活动被EA抑制。我们特别确定了一种强化的往复通路我们还定义了NRP作为处理来自vlPAG的谷氨酸输入的细胞核的重要作用，并通过5-HT1A rVLM机制调节交感神经流出。这种竞争性更新的中心假设是，标准化的针灸治疗过程和既定的方法将无反应转化为反应，以提高EA降低血压升高（BP）的疗效，特别是通过其对下丘脑室旁核（PVN）的抑制作用。目前的赠款将集中在四个新的领域：1)经弓形神经输入的阿片（脑啡肽和内啡肽）通过投射到rVLM和IML，在EA过程中调节PVN的作用，抑制反射诱发的交感神经激活；2)有意识冷致高血压（CI-HTN）大鼠重复EA降低血压的机制；3)在反复刺激EA降低CI-HTN大鼠血压时，调节rVLM中脑啡肽和内啡肽产生的转录机制；4)对EA无反应的动物PVN中胆囊收缩素对阿片活性的神经调节。药理学和分子方法将被用于与有可能从临床针灸获益的患者高度相关的拟议研究中。