HUMAN IMMUNOLOGY

约翰霍普金斯大学（JHU） Sidney Kimmel综合癌症中心（SKCCC）的癌症免疫学研究经历了从宿主对癌症免疫反应的基础实验室研究到基于免疫的癌症治疗的临床评估的自然进展。许多这些基于免疫的策略来调动和增强抗肿瘤免疫反应是建立在JHU研究人员的发现之上的。此外，在过去几年中，免疫治疗策略作为癌症治疗的辅助手段在各种恶性肿瘤和治疗环境中的应用已经急剧扩大。仔细和可靠地量化人体免疫反应的能力对于评估任何免疫治疗策略的影响至关重要。虽然正在研究的治疗方式有很大的多样性，但事实上，大多数领域在免疫学分析的技术和方法上都有很大的重叠。描述和量化人体免疫反应的现代免疫学技术是复杂的，单个研究人员很难将所有可能最适合某一特定项目的技术集合起来。人类免疫学核心实验室（HICL）成立于2005年，为SKCCC研究人员提供可靠的监测和定量人类免疫反应的能力，使用最先进的免疫分析。因此，本核心的具体目标是：1)提供技术专长并进行免疫学分析，以监测和表征人类免疫反应；2)建立所有免疫检测的标准操作程序和质量控制措施；3)向SKCCC调查人员传达其专业知识和可用性；4)为SKCCC研究人员提供技术支持，以确定和开发针对其研究目标的检测方法；5)作为对人类免疫学研究有用的关键试剂和细胞系的存储库。综上所述，HICL在监测人类免疫反应方面发挥着重要作用，这些免疫反应跨越了SKCCC内的许多学科，并提供了新的治疗方法来增强抗肿瘤免疫反应。Lay：人类免疫学核心实验室鉴定了参与癌症免疫反应的不同淋巴细胞亚群，并评估了它们的功能和它们产生炎症细胞因子的能力，炎症细胞因子放大了免疫反应。通过跟踪或监测免疫反应，人类免疫学核心实验室可以帮助研究人员评估其治疗的影响或影响。

Research in cancer immunology at the Sidney Kimmel Comprehensive Cancer Center (SKCCC), Johns Hopkins University (JHU) has undergone a natural progression from basic laboratory studies of the host immune response to cancer to the clinical evaluation of immune-based cancer therapies. Many of these immune-based strategies to mobilize and augment the anti-tumor immune response were founded on the discoveries made by JHU investigators. Moreover, the application of immunotherapeutic strategies as an adjunct to cancer treatment across a wide range of malignancies and therapeutic settings has expanded dramatically over the past several years. The ability to carefully and reliably quantify the human immune response is critically important to assess the impact of any immunotherapeutic strategy. While there is great diversity in the therapeutic modalities being studied, most areas, in fact, overlap strongly in their techniques and approaches to immunological analysis. Modern immunological techniques for characterizing and quantifying human immune responses are complex, and it is difficult for individual investigators to assemble all the techniques that may be most appropriate for a particular project. The Human Immunology Core Laboratory (HICL) was established in 2005 to provide SKCCC investigators the capacity to reliably monitor and quantify human immune responses using state-of-the-art immunologic assays. Thus, the specific goals of this CORE are to: 1) Provide technical expertise and conduct immunological assays to monitor and characterize the human immune responses; 2) Establish standard operating procedures and quality control measures for all immunological assays; 3) Communicate its expertise and availability to SKCCC investigators; 4) Provide technical support to SKCCC investigators seeking to identify and develop assays specific to their research objectives, and 5) Serve as a repository for key reagents and cell lines useful for the study of human immunology. Taken together, the HICL plays an important role in monitoring human immune responses that crosses many disciplines within the SKCCC and provides for the development of new therapeutic approaches to augment the anti-tumor immune response. Lay: The Human Immunology Core Laboratory identifies different subsets of lymphocytes that participate in the immune response to cancer and assesses their function and their ability to produce inflammatory cytokines that amplify the immune response. By tracking or monitoring the immune response, the Human Immunology Core Laboratory can assist investigators assess the impact or influence of their therapy.