FAST THREE-DIMENSIONAL IMAGING OF NEURONAL ACTIVITY AND PLASTICITY

神经元活动和可塑性的快速三维成像

• 批准号: 8294733
• 负责人: Timothy Holy
• 金额: $ 37.24万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-15 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8294733
• 关键词: Accessory Olfactory Bulbs; Brain; Computers; Coupled; Event; Future; Health; Image; Imaging Techniques; In Vitro; Learning; Life; Light; Lighting; Measures; Medical; Memory; Memory impairment; Methods; Microscopy; Monitor; Neuronal Plasticity; Neurons; Noise; Optics; Pattern; Phase; Physiological; Physiology; Plastics; Play; Population; Research; Resolution; Role; Sampling; Shapes; Signal Transduction; Software Tools; Speed; Stimulus; Synapses; System; Techniques; Thick; Three-Dimensional Imaging; Time; Tissues; Trees; Work; adaptive optics; base; experience; fluorescence imaging; fluorophore; improved; in vivo; instrument; memory encoding; millisecond; neurophysiology; new technology; novel; research study; spatiotemporal; stem

DESCRIPTION (provided by applicant): In studies of neural plasticity, one of the outstanding challenges is to connect our growing understanding of cellular and synaptic mechanisms to their consequences for plasticity of whole neuronal circuits. A promising approach is to use optical microscopy to observe the changes in activity that accompany learning; however, conventional methods like multiphoton microscopy cannot monitor neuronal events over large regions at high speeds. A new technique, Objective-Coupled Planar Illumination (OCPI) microscopy, promises orders-of- magnitude increases in the sensitivity and speed of optical physiology. This proposal focuses on extending the spatiotemporal resolution of OCPI microscopy to observe neuronal activity and plasticity at subcellular resolution in thousands of neurons simultaneously. PUBLIC HEALTH RELEVANCE: The mechanisms of learning are among the great unsolved mysteries of the brain, and memory deficits are a major medical problem. To better understand the neuronal basis of learning, we will develop new instruments for observing the changes in the brain during memory formation.

描述（由申请人提供）：在神经可塑性的研究中，突出的挑战之一是将我们对细胞和突触机制的不断增长的理解与其对整个神经元回路可塑性的影响联系起来。一种有前途的方法是使用光学显微镜来观察伴随学习的活动变化;然而，像多光子显微镜这样的传统方法无法高速监测大区域的神经元事件。一种新的技术，光耦合平面照明（OCPI）显微镜，承诺在光学生理学的灵敏度和速度的数量级增加。该建议的重点是扩展时空分辨率的OCPI显微镜观察神经元的活动和可塑性，同时在数千个神经元的亚细胞分辨率。公共卫生关系：学习的机制是大脑中最大的未解之谜之一，记忆缺陷是一个主要的医学问题。为了更好地理解学习的神经基础，我们将开发新的仪器来观察记忆形成过程中大脑的变化。