Flow Cytometry/Cell Sorting

流式细胞术/细胞分选

• 批准号: 8261979
• 负责人: GUANG-XIAN ZHANG
• 金额: $ 8.02万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8261979
• 关键词: Area; Autoimmunity; Cell Separation; Cells; Clinical; Clinical Trials; Collaborations; Color; Consult; Cytometry; Development; Flow Cytometry; Goals; Grant; Human Resources; Immune; Individual; Lasers; Literature; Phenotype; Philadelphia; Price; Protocols documentation; Publishing; Reagent; Recovery; Research; Research Personnel; Research Project Grants; Resources; Running; Services; Sorting - Cell Movement; Specialist; Speed; Sterility; Surface; System; Techniques; Technology; Time; Universities; cell type; member; research study; success; tool

The Flow Cytometry and Cell Sorting Core will provide standard technology and analysis services to the Philadelphia ACE investigators. This core will be used by both research projects, both clinical components and the pilot research project. It is non-duplicative of other services or facilities available to ACE investigators. The Core will be built around a state-of-the-art sorting system that has fixed alignment of the three laser beams (for the blue, red, and UV lasers), which can perform multi-color analysis (up to 13) in a single cell and simultaneously sort 4 subpopulations with high purity, high recovery, high speed, and sterile cell sorting, thus being essential for the experiments proposed in projects by the Pis. The core resources are not otherwise available at the Thomas Jefferson University or through other grant mechanisms. Two certified specialists, including the Core Director and one full-time research assistant, will routinely run this core to provide high-quality service. The goals of the Flow Cytometry and Cell Sorting Core are to: 1) Offer high quality and affordably priced services for analysis and sorting of a range of cell types, with particular emphasis on immune cells according to the needs of autoimmunity investigators in the ACE; 2) Consult with ACE members to determine their individual analytical cytometry needs, to aid in the development of appropriate protocols and techniques, giving advice for both standard and specialized services; 3) Promote interaction and collaboration among investigators by providing a centralized facility where exchange of results and ideas will be facilitated. As flow cytometry will be extensively used by all the Pis, this well-equipped core, in both machine and personnel, will provide a powerful tool for the overall success of this ACE.

流式细胞术和细胞分选核心将提供标准技术和分析服务 费城ACE调查人员。这一核心将用于两个研究项目，两个临床组成部分 和先导研究项目。它不与ACE提供的其他服务或设施重复 调查人员。 核心将围绕一种最先进的分类系统构建，该系统具有三个激光器的固定对准 光束(用于蓝、红和紫外光激光器)，可在单个单元中执行多色分析(最多13个 同时对4个亚群进行高纯度、高回收率、高速、无菌细胞分选， 因此对于PIS提出的项目中的实验是必不可少的。 核心资源不在托马斯·杰斐逊大学或通过其他赠款获得 机制。两名认证专家，包括核心主任和一名全职研究助理，将 例行公事地运行这个核心，提供高质量的服务。 流式细胞仪和细胞分选核心的目标是：1)提供高质量和实惠的价格 用于分析和分类一系列细胞类型的服务，特别强调免疫细胞，根据 以满足ACE中自身免疫调查人员的需求；2)咨询ACE成员以确定其 个体分析细胞术需要，以帮助开发适当的方案和技术， 为标准和专业服务提供建议；3)促进以下方面的互动和合作 通过提供一个便于交流成果和想法的集中化设施，调查人员的能力得到了提高。 由于流式细胞术将被所有PI广泛使用，这一配备良好的核心在机器和 这将为本届ACE的全面成功提供强有力的工具。