Flow Cytometry/Cell Sorting

流式细胞术和细胞分选核心将为 费城ACE调查员。该核心将用于两个研究项目，两个临床组成部分 和试点研究项目。它不重复ACE提供的其他服务或设施 investigators. 核心将建立在一个国家的最先进的分拣系统，具有固定的三个激光对准 光束（用于蓝色、红色和UV激光器），可在单个池中执行多色分析（最多13种） 同时分选4个亚群，高纯度、高回收率、高速度、无菌细胞分选， 因此对于Pis在项目中提出的实验至关重要。 核心资源是不以其他方式在托马斯杰斐逊大学或通过其他赠款 机制等两名认证专家，包括核心主任和一名全职研究助理，将 定期运行此核心以提供高质量的服务。 流式细胞仪和细胞分选核心的目标是：1）提供高质量和实惠的价格 提供一系列细胞类型的分析和分选服务，特别强调免疫细胞， ACE中自身免疫研究者的需求; 2）与ACE成员协商，以确定他们的 个体分析细胞术需要，以帮助开发适当的方案和技术， 为标准和专业服务提供咨询; 3）促进 通过提供一个便于交流成果和想法的中央设施，促进调查人员的交流。 由于流式细胞术将被所有的PI广泛使用，这个装备精良的核心，在机器和 人员，将提供一个强有力的工具，这一ACE的整体成功。

The Flow Cytometry and Cell Sorting Core will provide standard technology and analysis services to the Philadelphia ACE investigators. This core will be used by both research projects, both clinical components and the pilot research project. It is non-duplicative of other services or facilities available to ACE investigators. The Core will be built around a state-of-the-art sorting system that has fixed alignment of the three laser beams (for the blue, red, and UV lasers), which can perform multi-color analysis (up to 13) in a single cell and simultaneously sort 4 subpopulations with high purity, high recovery, high speed, and sterile cell sorting, thus being essential for the experiments proposed in projects by the Pis. The core resources are not otherwise available at the Thomas Jefferson University or through other grant mechanisms. Two certified specialists, including the Core Director and one full-time research assistant, will routinely run this core to provide high-quality service. The goals of the Flow Cytometry and Cell Sorting Core are to: 1) Offer high quality and affordably priced services for analysis and sorting of a range of cell types, with particular emphasis on immune cells according to the needs of autoimmunity investigators in the ACE; 2) Consult with ACE members to determine their individual analytical cytometry needs, to aid in the development of appropriate protocols and techniques, giving advice for both standard and specialized services; 3) Promote interaction and collaboration among investigators by providing a centralized facility where exchange of results and ideas will be facilitated. As flow cytometry will be extensively used by all the Pis, this well-equipped core, in both machine and personnel, will provide a powerful tool for the overall success of this ACE.