Microengineered Osteons for Bone Tissue Engineering

描述(由申请人提供)： 微工程化骨用于骨组织工程修复承重骨缺损仍然是一个重要的临床问题。骨重建手术通常需要骨移植，以帮助骨折愈合、骨融合、种植体整合和修复骨缺损，但同种异体骨的使用受到高失败率的限制。组织工程构建是很有前途的替代品。然而，用于骨修复的工程化组织往往受到缺乏血管、难以构建复杂的骨微结构以及支架机械强度不足等因素的限制。我们建议使用一种多尺度的方法来进行骨组织工程，将纳米级分子排列、微工程组织和仿生骨支架相结合。因此，我们的目标是生成微工程化的骨样单位，并将得到的结构整合到仿生支架中，并在体外和体内分析这些结构。这将导致矿化血管组织在各种功能和生理相关的尺度上具有受控的结构。预计该项目将通过在皮质骨中形成复杂的微结构，如具有血管通道的骨样单位，从而在骨组织工程中产生新的范例。 公共卫生相关性： 负荷性骨缺损的修复仍然是一个巨大的临床挑战。该项目有望在骨组织工程中产生一种新的范例，通过在皮质骨中形成复杂的微结构，如具有血管通道的骨样单位。

DESCRIPTION (provided by applicant): Microengineered osteons for bone tissue engineering Repair of load bearing bone defects remains a significant clinical problem. Often bone grafts are required for reconstructive skeletal procedures to aid fracture healing, bone fusion, implant integration and repair of skeletal defects, but the use of allograft bone is limited by a high failure rate. Tissue engineered constructs are promising substitutes. However, engineered tissues for bone repair are often limited by a lack of vascularity, the difficulty to engineer the complex bone microarchitecture and insufficient scaffold mechanical strength. We propose to use a multi-scale approach to bone tissue engineering that combines nanoscale molecular alignment, microengineered tissues and biomimetic bone scaffolds. We therefore aim to generate microengineered osteon-like units and to integrate the resulting structures within biomimetic scaffolds and to analyze the constructs in vitro and in vivo. This will result in mineralized vascular tissues with controlled architectures on a variety of functional and physiologically relevant scales. It is expected that this project will generate a new paradigm in bone tissue engineering by enabling the formation of complex microstructures such as osteon-like units with vascular channels within cortical bone. PUBLIC HEALTH RELEVANCE: Repair of load bearing bone defects remains a significant clinical challenge. This project is expected to generate a new paradigm in bone tissue engineering by enabling the formation of complex microstructures such as osteon-like units with vascular channels within cortical bone.