Microengineered Osteons for Bone Tissue Engineering

用于骨组织工程的微工程骨

• 批准号: 8517582
• 负责人: YUNZHI YANG
• 金额: $ 41.79万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8517582
• 关键词: Address; Allografting; Architecture; Area; Biocompatible Materials; Biomechanics; Biomimetics; Blood Vessels; Bone Marrow; Bone Regeneration; Bone Tissue; Bone Transplantation; Calculi; Cell Communication; Cells; Clinical; Collagen; Collagen Fiber; Complex; Defect; Development; Endothelial Cells; Engineering; Extracellular Matrix; Failure; Fracture; Fracture Healing; Gel; Generations; Germ Cells; Goals; Growth Factor; Human; Hydrogels; Immobilization; Implant; In Vitro; Limb structure; Maintenance; Mechanics; Metabolic; Microfabrication; Molecular; Molecular Biology; Natural regeneration; Nutrient; Orthopedic Surgery procedures; Osteoblasts; Osteogenesis; Osteon; Pattern; Performance; Porosity; Procedures; Production; Property; Resolution; Structure; Techniques; Technology; Testing; Tissue Engineering; Tissues; United States; Vascular Endothelial Growth Factors; Weight-Bearing state; Work; abstracting; allogenic bone transplantation; bone; bone morphogenetic protein 2; crosslink; improved; in vivo; long bone; meetings; mineralization; nano; nanoengineering; nanoscale; osteogenic; prevent; repaired; response; scaffold; skeletal; skeletal tissue; tissue regeneration; tissue repair; tricalcium phosphate

Project abstract: Microengineered osteons for bone tissue engineering Repair of load bearing bone defects remains a significant clinical problem. Often bone grafts are required for reconstructive skeletal procedures to aid fracture healing, bone fusion, implant integration and repair of skeletal defects, but the use of allograft bone is limited by a high failure rate. Tissue engineered constructs are promising substitutes. However, engineered tissues for bone repair are often limited by a lack of vascularity, the difficulty to engineer the complex bone microarchitecture and insufficient scaffold mechanical strength. We propose to use a multi-scale approach to bone tissue engineering that combines nanoscale molecular alignment, microengineered tissues and biomimetic bone scaffolds. We therefore aim to generate microengineered osteon-like units and to integrate the resulting structures within biomimetic scaffolds and to analyze the constructs in vitro and in vivo. This will result in mineralized vascular tissues with controlled architectures on a variety of functional and physiologically relevant scales. It is expected that this project will generate a new paradigm in bone tissue engineering by enabling the formation of complex microstructures such as osteon-like units with vascular channels within cortical bone.

项目摘要： 骨组织工程中的微工程骨单位 承重骨缺损的修复仍然是一个重要的临床问题。通常需要进行骨移植 用于重建骨骼手术，以帮助骨折愈合、骨融合、植入物整合和修复 骨缺损，但使用同种异体骨是有限的高失败率。组织工程构建体 是很有前途的替代品。然而，用于骨修复的工程化组织通常受到缺乏生物相容性的限制。 血管丰富，复杂的骨微结构难以工程化，支架不足 机械强度我们建议使用多尺度方法进行骨组织工程， 纳米级分子排列、微工程组织和仿生骨支架。因此，我们的目标是 以产生微工程化的骨样单位，并将所得结构整合到仿生学中， 支架，并在体外和体内分析构建体。这将导致矿化的血管组织 具有各种功能和生理相关尺度上的受控结构。预计 该项目将通过形成骨组织工程学的新范式， 复杂的微观结构，如皮质骨内具有血管通道的骨样单位。