IMAGING & HOSTOPATH

影像/组织病理学模块是解剖学/细胞生物学系的一个集中设施， 提供光学、激光共聚焦扫描和电子显微镜专业知识，优先使用NEI资助 核心视觉科学家。该模块已通过核心拨款、部门、 机构和个人R01支持，并提供广泛的功能，不仅将增强 目前由NEI资助的工作，但这将促进新的供资机会和合作，这是显而易见的 下面。 该模块包含两个大型设备，JEOL 1010变速器EM和NEI获奖 徕卡TSC SP2共聚焦激光扫描显微镜(后者是上届参赛以来新增的 核心补助金的续期)。该模块位于斯科特·霍尔的两层楼内。1900平方英尺。英国《金融时报》设施位于 Scott Hall的7341、7345、7347、7350、7354室。本网站提供共聚焦激光扫描和 电子显微镜、组织加工和切片、免疫化学和裂隙灯摄影。8号 楼层设有另一套模块专用客房，面积为1,200平方米。FT空间(8344、8350、 8352和8354)。这一领域包括蔡司Axiophote和蔡司Axiocam的图像处理和分析 显微镜系统以及蔡司Apotome显微镜。该区域还包括计算机工作站 区域，3台Macintosh和5台PC计算机，平板扫描仪功能，8台打印机和各种软件 (包括变形器)。为调查人员提供一流的技术支持、设备监督和培训 由经验丰富的研究助理罗纳德·巴雷特先生(7楼)和王耀英女士(8楼)主持。 模块内的协作方式多种多样。最基本的是，该模块提供了熟练的 向NEI资助的个人提供技术专长和维护良好的设备，这些人主要 形态学者。例如，眼科医生可能需要通过形态或其他方式进行记录 摄影方法他感兴趣的细胞群分离和表征或在 核心组织培养设施。该模块为这一需求提供的援助类型是简单的，但 对那个人来说很重要的水平。协作还可以包括在实验方案方面的协助 开发，确定要使用的适当固定剂，形态数据的解释， 摄影人物准备、数字图形应用、海报和幻灯片制作，最后是 在稿件提交方面进行合作的可能性。这也是该模块的目标是加强，在较低的 那些通过提供技术援助寻求NEI资金的人的优先事项、协作和生产力， 设备的可用性以及与不同领域的视觉研究人员的互动。这些类型的协作 和互动目前由该设施提供，模块联席主任的目标是继续 以鼓励和扩大它们。

The Imaging/Histopathology Module is a centralized facility in the Anatomy/Cell Biology Department that provides light, confocal laser scanning, and electron microscopic expertise with priority of use to NEI funded Core vision scientists. The Module has been expanded and enhanced with Core grant, Departmental, Institutional, and individual R01 support and offers a wide range of capabilities which will not only enhance ongoing NEI-funded work but which will facilitate new funding opportunities and collaborations as evidenced below. The Module contains two large pieces of equipment, the JEOL 1010 transmission EM and the NEI awarded Leica TSC SP2 confocal laser scanning microscope (the latter is newly added since the last competing renewal of the Core grant). The module is housed on two floors of Scott Hall. A 1900 sq. ft. facility is located in rooms 7341, 7345, 7347, 7350, 7354 in Scott Hall and this site provides for confocal laser scanning and electron microscopy, tissue processing and sectioning, immunochemistry, and slit lamp photography. The 8th floor houses the other suite of Module dedicated rooms and includes 1,200 sq. ft of space (rooms 8344, 8350, 8352 and 8354). This area includes the Zeiss Axiophot and Zeiss Axiocam image processing and analysis microscope system as well as the Zeiss Apotome microscope. The area also includes computer workstation areas, 3 MacinTosh and 5 PC computers, flat bed scanner capability, 8 printers and a variety of software (including Metamorph). Superb technical support, equipment oversight and training to investigators is provided by skilled research assistants, Mr. Ronald Barrett (7th floor) and Mrs. Yaoying Wang (8th floor). Collaboration within the Module occurs in a variety of ways. Most basically, the Module provides skilled technical expertise and well-maintained equipment to NEI funded individuals who are not primarily morphologists. For example, an ophthalmologist may need to document by morphological or other photographic methods a cell population that he is interested in isolating and characterizing or growing in the Core tissue culture facility. The type of assistance provided by the Module for this need is at a simple, yet important level to that individual. Collaboration may also consist of assistance in experimental protocol development, determining appropriate fixatives to be utilized, interpretation of morphological data, photographic figure preparation, digital graphics applications, poster and slide production, and lastly, the possibility of collaboration in manuscript submission. It is also the goal of the Module to enhance, at lower priority, collaboration and productivity of those who are seeking NEI funding by providing technical assistance, equipment availability and interaction with vision researchers of diverse fields. These types of collaborations and interactions are currently provided for by the facility and it is the goal of the Module co-directors to continue to encourage and expand them.