IMAGING & HOSTOPATH

影像学

• 批准号: 8614809
• 负责人: FU-SHIN YU
• 金额: $ 5.42万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8614809
• 关键词: Anatomy; Animals; Applications Grants; Area; Award; Beds; Biochemistry; Cancer Center Support Grant; Cells; Cellular biology; Collaborations; Computer Graphics; Computer Workstations; Computer software; Computers; Data; Digital Photography; Electron Microscopy; Electrons; Equipment; Fixatives; Floor; Freezing; Funding; Funding Opportunities; Goals; Histopathology; Housing; Image; Image Analysis; Immunochemistry; Immunofluorescence Immunologic; Immunology; Individual; Lasers; Light; Link; Manuscripts; Methods; Microbiology; Microscope; Microscopic; Microscopy; Microtome - medical device; Molecular Biology; Morphology; Names; Neurosciences; Ophthalmologist; Outcome; Photography; Population; Preparation; Production; Productivity; Publications; Published Comment; Research; Research Personnel; Research Training; Resolution; Scanning; Scanning Electron Microscopy; Scientist; Site; Slide; System; Talents; Technical Expertise; Tissues; Training; Vision; Vision research; Work; base; digital; digital imaging; image processing; interest; light microscopy; meetings; posters; protocol development; tissue culture; tissue processing; transmission process

The Imaging/Histopathology Module is a centralized facility in the Anatomy/Cell Biology Department that provides light, confocal laser scanning, and electron microscopic expertise with priority of use to NEI funded Core vision scientists. The Module has been expanded and enhanced with Core grant, Departmental, Institutional, and individual R01 support and offers a wide range of capabilities which will not only enhance ongoing NEI-funded work but which will facilitate new funding opportunities and collaborations as evidenced below. The Module contains two large pieces of equipment, the JEOL 1010 transmission EM and the NEI awarded Leica TSC SP2 confocal laser scanning microscope (the latter is newly added since the last competing renewal of the Core grant). The module is housed on two floors of Scott Hall. A 1900 sq. ft. facility is located in rooms 7341, 7345, 7347, 7350, 7354 in Scott Hall and this site provides for confocal laser scanning and electron microscopy, tissue processing and sectioning, immunochemistry, and slit lamp photography. The 8th floor houses the other suite of Module dedicated rooms and includes 1,200 sq. ft of space (rooms 8344, 8350, 8352 and 8354). This area includes the Zeiss Axiophot and Zeiss Axiocam image processing and analysis microscope system as well as the Zeiss Apotome microscope. The area also includes computer workstation areas, 3 MacinTosh and 5 PC computers, flat bed scanner capability, 8 printers and a variety of software (including Metamorph). Superb technical support, equipment oversight and training to investigators is provided by skilled research assistants, Mr. Ronald Barrett (7th floor) and Mrs. Yaoying Wang (8th floor). Collaboration within the Module occurs in a variety of ways. Most basically, the Module provides skilled technical expertise and well-maintained equipment to NEI funded individuals who are not primarily morphologists. For example, an ophthalmologist may need to document by morphological or other photographic methods a cell population that he is interested in isolating and characterizing or growing in the Core tissue culture facility. The type of assistance provided by the Module for this need is at a simple, yet important level to that individual. Collaboration may also consist of assistance in experimental protocol development, determining appropriate fixatives to be utilized, interpretation of morphological data, photographic figure preparation, digital graphics applications, poster and slide production, and lastly, the possibility of collaboration in manuscript submission. It is also the goal of the Module to enhance, at lower priority, collaboration and productivity of those who are seeking NEI funding by providing technical assistance, equipment availability and interaction with vision researchers of diverse fields. These types of collaborations and interactions are currently provided for by the facility and it is the goal of the Module co-directors to continue to encourage and expand them.

成像/组织学模块是解剖学/细胞生物学系的一个集中设施， 提供光、共聚焦激光扫描和电子显微镜技术，优先用于NEI资助的项目。 核心视觉科学家。该单元已得到扩大和加强，核心赠款，部门， 机构和个人R 01的支持，并提供了广泛的能力，这不仅将提高 正在进行的NEI资助的工作，但这将促进新的资助机会和合作， 下面 该模块包含两个大型设备，JEOL 1010变速器EM和NEI Leica TSC SP2共聚焦激光扫描显微镜（后者是自上一次竞争后新增加的 核心补助金的更新）。该模块位于Scott Hall的两层楼。1900平方米英尺设施位于 房间7341，7345，7347，7350，7354在斯科特大厅和这个网站提供共焦激光扫描， 电子显微镜、组织处理和切片、免疫化学和裂隙灯摄影。第8 楼层设有模块专用客房的其他套房，包括1，200平方米。英尺的空间（8344，8350， 8352和8354）。该区域包括Zeiss Axiophot和Zeiss Axiocam图像处理和分析 显微镜系统以及蔡司Apotome显微镜。该区域还包括计算机工作站 区域，3台MacinTosh和5台PC计算机，平板扫描仪能力，8台打印机和各种软件 （包括Metamorph）为调查人员提供一流的技术支持、设备监督和培训 由技术娴熟的研究助理罗纳德巴雷特先生（7楼）和王耀英女士（8楼）。 模块内的协作以各种方式进行。最基本的是，该模块提供熟练的 技术专长和维护良好的设备，以NEI资助的个人谁不是主要 形态学家例如，眼科医生可能需要通过形态学或其他方法来记录 摄影方法他感兴趣的细胞群的分离和表征或在细胞中生长 核心组织培养设施。该单元为这一需要提供的援助类型简单，但 对个人来说很重要。合作还可包括协助制定实验方案 显影，确定要使用的适当固定剂，解释形态学数据， 摄影人物准备，数字图形应用，海报和幻灯片制作，最后， 合作投稿的可能性。该模块的另一个目标是， 通过提供技术援助寻求NEI资金的人的优先权、合作和生产力， 设备的可用性和与不同领域的视觉研究人员的互动。这些类型的合作 和互动目前是由设施提供的，这是模块共同主任的目标，继续 鼓励和扩大他们。