AB SCIEX 5500 QTRAP Mass Spectrometer for Lipidomics and Metabolomics Research

用于脂质组学和代谢组学研究的 AB SCIEX 5500 QTRAP 质谱仪

• 批准号: 8246785
• 负责人: CHARLES E. CHALFANT
• 金额: $ 57.78万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-08 至 2013-05-07
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246785
• 关键词: Advisory Committees; Cell physiology; Complex; Core Facility; Development; Environment; Equipment; Funding; Human; Ions; Lipids; Metabolism; Minor; N.I.H. Research Support; Organelles; Patients; Research; Research Personnel; Research Project Grants; Resources; Sample Size; Sampling; Scientist; Signal Pathway; Signal Transduction; System; United States National Institutes of Health; Universities; Virginia; experience; human disease; instrument; ion source; lipid metabolism; mass spectrometer; metabolomics; programs

DESCRIPTION (provided by applicant): Virginia Commonwealth University (VCU) has a very active and growing critical mass of funded investigators examining how dynamic changes in bioactive lipids influence metabolism and the signaling pathways that regulate normal and pathological human cellular functions. Funds are requested for the acquisition of the Applied Biosystems/SCIEX 5500 Quadrupole Linear Ion Trap Mass Spectrometer with electrospray and nanospray ion sources (5500 QTRAP) that will enhance the analysis capabilities of the Lipidomics/Metabolomics core facility. This new mass spectrometer system will dramatically increase the our ability to obtain more information from complex samples, increase qualitative and quantitative analysis of low abundance, bioactive lipids, drastically reduce sample size requirements important for patient samples, and finally, will allow researchers to investigate localized lipid metabolism and function in subcellular organelles. As described in this application, 7 NIH-funded scientists and clinicians, major users with over $7 M NIH supported research programs, and 10 minor users from 8 different departments have specific needs for this equipment. The 5500 QTRAP system together with the existing QTRAPs will be operated as a shared research resource to provide essential support for our ongoing research programs and to facilitate new collaborative research initiatives for lipid identification, quantitation, an characterization, with particular emphasis on cell signaling and functional lipidomics. These needs for enhanced sensitivity, analysis of more complex lipids and metabolites, smaller sample sizes, and increasing demand from more research projects provide the principal justification for this request. The instrument will be administered as a VCU core resource under the technical direction of Dr. Jeremy Allegood, who has extensive technical experience in lipid analysis and administration of core resources. Drs. Charles Chalfant (the PI) and Sarah Spiegel will serve as co- Scientific Directors and will serve on the Internal Advisory Committee together with major users from other departments to oversee equitable use of the instrument for the benefit of NIH-funded investigators and the university research environment. The purchase of this equipment is essential to continuing development and realization of the full potential of lipidomics and metabolomics research toward better understanding of normal cellular function and human disease at VCU. PUBLIC HEALTH RELEVANCE: Virginia Commonwealth University (VCU) has a very active and growing critical mass of funded investigators examining how dynamic changes in bioactive lipids influence metabolism and the signaling pathways that regulate normal and pathological human cellular functions. Funds are requested for the acquisition of a new mass spectrometer system (e.g. Applied Biosystems/SCIEX 5500 Quadrupole Linear Ion Trap Mass Spectrometer with electrospray and nanospray ion sources) to dramatically enhance the analysis capabilities of the Lipidomics/Metabolomics core facility at VCU to aid NIH-funded investigators undertake scientific research toward curing human diseases.

描述（由申请人提供）：弗吉尼亚联邦大学 (VCU) 拥有一批非常活跃且不断增长的资助研究人员，研究生物活性脂质的动态变化如何影响新陈代谢以及调节正常和病理性人类细胞功能的信号通路。申请资金用于购买带有电喷雾和纳喷雾离子源 (5500 QTRAP) 的 Applied Biosystems/SCIEX 5500 四极线性离子阱质谱仪，这将增强脂质组学/代谢组学核心设施的分析能力。这种新的质谱仪系统将极大地提高我们从复杂样品中获取更多信息的能力，增加对低丰度、生物活性脂质的定性和定量分析，大大减少对患者样品重要的样品量要求，最后，将使研究人员能够研究亚细胞细胞器中的局部脂质代谢和功能。如本申请中所述，7 位 NIH 资助的科学家和临床医生、NIH 支持的研究项目超过 700 万美元的主要用户以及来自 8 个不同部门的 10 位次要用户对此设备有特定需求。 5500 QTRAP 系统与现有的 QTRAP 将作为共享研究资源运行，为我们正在进行的研究项目提供必要的支持，并促进脂质鉴定、定量、表征的新合作研究计划，特别强调细胞信号传导和功能脂质组学。对提高灵敏度、分析更复杂的脂质和代谢物、更小的样本量以及更多研究项目不断增加的需求的需求为这一要求提供了主要理由。该仪器将作为 VCU 核心资源在 Jeremy Allegood 博士的技术指导下进行管理，他在脂质分析和核心资源管理方面拥有丰富的技术经验。博士。 Charles Chalfant（PI）和 Sarah Spiegel 将担任联合科学主任，并将与其他部门的主要用户一起在内部咨询委员会任职，以监督该仪器的公平使用，以造福 NIH 资助的研究人员和大学研究环境。购买该设备对于继续开发和实现脂质组学和代谢组学研究的全部潜力至关重要，以更好地了解弗吉尼亚联邦大学的正常细胞功能和人类疾病。 公共健康相关性：弗吉尼亚联邦大学 (VCU) 拥有一批非常活跃且不断增长的资助研究人员，研究生物活性脂质的动态变化如何影响新陈代谢以及调节正常和病理性人类细胞功能的信号通路。需要资金购买新的质谱仪系统（例如带有电喷雾和纳喷雾离子源的 Applied Biosystems/SCIEX 5500 四极杆线性离子阱质谱仪），以显着增强 VCU 脂质组学/代谢组学核心设施的分析能力，以帮助 NIH 资助的研究人员开展治愈人类疾病的科学研究 疾病。