A spinning disk confocal microscope for live-cell imaging

用于活细胞成像的转盘共焦显微镜

• 批准号: 8247453
• 负责人: Anthony Brown
• 金额: $ 59.98万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247453
• 关键词: Appointment; Award; Axonal Transport; Cells; Cellular biology; Center Core Grants; Computer software; Core Facility; Cytoskeleton; DNA Sequence; DNA Sequencing Facility; Electron Microscopy; Equipment; Faculty; Funding; Grant; Housing; Image; Joints; Laboratory Research; Life; Maintenance; Malignant Neoplasms; Master' s Degree; Microscope; Minor; Molecular Neurobiology; Mus; Neurons; Neurosciences; Ohio; Research; Research Project Grants; Research Support; Resources; Running; Scanning; Schedule; Technology; Training; United States National Institutes of Health; Universities; Wages; Zebrafish; cellular imaging; college; embryo tissue; experience; light microscopy; new technology; operation; professor; programs; ranpirnase; tissue/cell culture

DESCRIPTION (provided by applicant): We are requesting funds to purchase a PerkinElmer UltraVIEW spinning disk confocal microscope for live imaging of cultured cells, tissues and embryos. The microscope will be housed in the Imaging Core Facility of the Center for Molecular Neurobiology on the West Campus of Ohio State University. Established about 25 years ago, the Center is a dynamic and interactive group of 13 faculty who have joint appointments in various Colleges. The Center has its own Director and support staff and excellent space and resources, including an adjacent mouse facility and a zebrafish core facility. The spinning disk confocal microscope purchased with this award represents critical and enabling technology that will support the research of 7 Center faculty (4 major users and 3 minor users) who are studying problems in neuroscience and cancer, and who collectively hold 9 active NIH grants totaling more than $2.4 million dollars in annual NIH funding. The Imaging Core Facility was established 6 years ago by Dr. Anthony Brown (the PI of this proposal) with funds from an NIH P30 Institutional Center Core Grant. The facility consists of a Leica TCS SL single-point scanning confocal microscope and a Zeiss Axiophot epifluorescence microscope with camera and software. Dr. Brown is the Director of the facility and supervises the Manager of the facility, Ms. Paula Monsma. Ms. Monsma manages al day-to-day operations, including training, user support, scheduling and equipment maintenance. Dr. Brown is a Professor in the Center for Molecular Neurobiology and an expert in live-cell imaging with more than 25 years of experience using light and electron microscopy in his research. Dr. Brown maintains an active and productive research program involving live-cell imaging of axonal transport and the cytoskeleton of nerve cells and has been funded by the NIH continuously for the past 15 years. Ms. Monsma has a Masters degree in cell biology and 15 years experience as a laboratory research assistant, including 5 years running a DNA sequencing core facility and 6 years running the Imaging Core Facility in the Center for Molecular Neurobiology. The P30 Center Core grant, which was recently renewed for another 5 years, provides 45% salary support for Ms. Monsma, enabling her to devote 2-3 days a week to her Imaging Core facility duties. In summary, this proposal leverages the expertise and resources established by our P30 Institutional Center Core Grant to acquire a critical and enabling new technology that will support the live-cell confocal imaging needs of 9 NIH-funded research projects in neuroscience and cancer. PUBLIC HEALTH RELEVANCE: Modern biomedical research is now critically dependent on direct observations of fluorescent molecules in living cells, tissues and organisms. This grant wil fund the purchase of an advanced state-of-the-art confocal fluorescence microscope for this purpose. The shared instrument will form part of the Imaging Core Facility in the Center for Molecular Neurobiology at Ohio State University, and it will provide vital new technological capabilities to support the research of 9 NIH-funded research projects in neuroscience and cancer.

描述（由申请人提供）：我们要求资金购买Perkinelmer Ultraview Spinning Disk共聚焦显微镜，以用于培养的细胞，组织和胚胎的实时成像。显微镜将安置在俄亥俄州立大学西校园分​​子神经生物学中心的成像核心设施中。该中心成立于大约25年前，是一个由13位教职员工组成的充满活力和互动的团体，他们在各种大学中有共同任命。该中心拥有自己的董事和支持人员，以及出色的空间和资源，包括相邻的老鼠设施和斑马鱼核心设施。该奖项购买的旋转磁盘共聚焦显微镜代表着关键而有能力的技术，该技术将支持研究神经科学和癌症问题的7位中心教师（4位主要用户和3个未成年人），他们共同持有9个活跃的NIH赠款，总计超过240万美元的NIH NIH资助。成像核心设施由6年前由Anthony Brown博士（该提案的PI）建立，并拥有NIH P30机构中心核心赠款的资金。该设施由Leica TCS SL单点扫描共聚焦显微镜和带相机和软件的Zeiss Axiophot Epifluorsince显微镜组成。布朗博士是该设施的主任，并监督该设施的经理Paula Monsma女士。 Monsma女士管理AL日常操作，包括培训，用户支持，日程安排和设备维护。 Brown博士是分子神经生物学中心的教授，也是活细胞成像专家，在他的研究中使用光和电子显微镜具有超过25年的经验。布朗博士维持了一项积极且富有生产力的研究计划，涉及轴突运输的活细胞成像和神经细胞的细胞骨架，并在过去15年中一直由NIH提供资金。 Monsma女士拥有细胞生物学硕士学位，并担任实验室研究助理15年的经验，其中包括经营DNA测序核心设施5年，并在分子神经生物学中心进行成像核心设施6年。最近续签了P30中心核心格兰特（Center Core Grant），又续签了5年，为Monsma女士提供了45％的薪水支持，使她每周2-3天能够将其成像核心设施职责。总而言之，该提案利用了我们的P30机构中心核心赠款建立的专业知识和资源，以获取一项至关重要的新技术，该技术将支持9个NIH资助的神经科学和癌症的9个基金资助的研究项目。 公共卫生相关性：现代生物医学研究现在严重取决于对活细胞，组织和生物体中荧光分子的直接观察。为此，这笔赠款资助了购买先进的最新共聚焦荧光显微镜。该工具将构成俄亥俄州立大学分子神经生物学中心成像核心设施的一部分，它将提供至关重要的新技术能力，以支持对NIH资助的NIH资助的神经科学和癌症研究项目的研究。