Bioavailavility & Bioanalytical Core/Weaver, Connie

核心B的目的是提供集中的生物分析和生物利用度资源，以 支持个别中心项目。核心B是通过促进以下方面的特点直接支持项目 葡萄多酚组分及其生物利用度、代谢和分布的测定 多酚对包括大脑在内的组织具有靶向作用。核心B对中心的执行至关重要 项目并雇佣了普渡大学、伊利诺伊大学和罗格斯大学提供的广泛专业知识 与现有的普渡-UAB植物中心相关联。核心B将被分为分子 成分-生物分析和生物利用度&代谢成分，以表征，存档 并评估单个项目中葡萄部分的体内生物利用度。分子组成& 生物分析组件将提供材料认证和葡萄多酚定量使用 LC/MS方法(Simon和Wu，Rutgers)。在通过生物测定确定特定多酚组分的优先顺序之后 引导分级，核心将提供LC-MS/MS(Simon和Wu，Rutgers)和核磁共振方面的专业知识 葡萄源多酚在生物活性组分中的表征及归档 在项目期内的服务。(项目1和项目3)。生物利用度与代谢 核心B组件将使用啮齿动物评估葡萄多酚的吸收、代谢和组织特征 该小组首创的模型、自动采血和膜探头(项目1、2和3， 珍尔和韦弗，普渡)。核心还将负责生产(Lila，UIUC)和行政管理 (普渡)的14C标记的葡萄分级(项目1和3)，然后分析活体样品 加速器质谱仪(AMS)技术跟踪痕量葡萄多酚和 它们的代谢物进入靶组织(项目1、2和3)。血药动学和血药浓度测定 未标记的多酚和代谢物的组织图谱将通过LC-MS/MS和LC-核磁共振完成 (项目1、2和3，Ademec、Raftery和Ferruzzi、普渡)。葡萄多酚在靶组织中的分析 而鉴定新的代谢物是了解它们与以下方面的生物学影响的基础 阿尔茨海默氏症的病理机制。核心B提供的信息将提供关于 葡萄衍生的多酚与阿尔茨海默病风险和预后的调节有关。

The purpose of Core B is to provide a centralized source of Bioanalytical and Bioavailability resources to support individual center projects. Core B is to directly support projects by facilitating characterization of grape derived polyphenol fractions and to determine bioavailability, metabolism and distribution of grapederived polyphenols to target tissues including brain. Core B is critically important for execution of the center projects and employs a wide range of expertise available from Purdue, University of Illinois, and Rutgers associated with the existing Purdue-UAB Botanicals Center. Core B will be divided into Molecular Composition & Bioanalytical and Bioavailability & Metabolism components in order to characterize, archive and assess in vivo bioavailability of grape fractions for individual projects. The Molecular Composition & Bioanalytical Component will provide material authentication and grape polyphenol quantification using the LC/MS methods (Simon and Wu, Rutgers). Following prioritization of specific polyphenol fractions by bioassay guided fractionation, the Core will provide expertise in LC-MS/MS (Simon and Wu, Rutgers) and NMR (Raftery, Purdue) characterization of grape-derived polyphenols in bioactive fractions as well as archiving services for the duration of the project period. (Projects 1 and 3). The Bioavailability & Metabolism Component of Core B will assess grape polyphenol absorption, metabolism and tissue profiles using rodent models, automated blood sampling and membrane probes pioneered by this group (Projects 1,2 and 3, Janle and Weaver, Purdue). The Core will also be responsible for production (Lila, UIUC) and administration (Janle, Purdue) of 14C labeled grape fractions (Projects 1 and 3) followed by analysis of in vivo samples by accelerator mass spectrometry (AMS) techniques to track trace amounts of grape derived polyphenols and their metabolites into target tissues (Project 1,2 and 3). Determination of plasma pharmacokinetics and tissue profiles for unlabeled polyphenols and metabolites will be accomplished by LC-MS/MS and LC-NMR (Projects 1, 2 and 3, Ademec, Raftery and Ferruzzi, Purdue). Analysis of grape polyphenols in target tissues and identification of novel metabolites is fundamental to understanding their biological impact in relation to Alzheimer's pathomechanisms. Information provided by Core B will provide critical knowledge on the fate of grape derived polypohenols associated with modulation of Alzheimer's disease risk and outcomes.