DIFFERENTIAL TROPHOBLAST FORMATION FROM RHESUS EMBRYONIC STEM CELLS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 目的：探讨子宫内膜对抗子宫内膜异位症的免疫形态学反应， 在恒河猴中模拟胚胎移植（MET）的程序。 进展： 尽管近几十年来IVF技术取得了重大进展， 植入率仍然很低。因为不可能直接进行 人类妊娠早期胚胎侵袭的形态生理学研究 生殖道的相似性使恒河猴成为一种有价值的动物模型 用于人体胚胎与 子宫内膜在着床过程中。 本研究包括四个动物组：1）一个动物输卵管和两个动物 用transcerebral MET，使用恒河猴ES细胞衍生的胚状体（EB转移）; 2） 2只动物接受仅含EB-培养基的transcerebral MET（假转移）; 3）2只 用孕酮处理而不使用MET的动物;和4）一只动物作为未处理的 控制在注射后第9或10天对动物实施安乐死（第21天一只猴）。 转移我们对白细胞亚群和内皮细胞进行了免疫组化， 标记物和PAS染色与血管和一般子宫内膜形态计量学。 免疫染色显示NK细胞（CD 56+和穿孔素+）数量减少， 所有子宫内膜上层CD 68+巨噬细胞和中性粒细胞 实验组与未处理的对照组比较。的这些变化 白细胞亚群与基质细胞密度的降低一致， 子宫内膜厚度和血管分布。在基底子宫内膜中， 穿孔素+细胞在所有动物中增加，而T细胞在猴子中减少， MET.令人惊讶的是，DC-SIGN+细胞在大多数（但不是全部） MET动物，尽管在其他组中这些细胞不存在。 我们的结论是，恒河猴可以被认为是一个潜在的模型， 监测子宫内膜容受性和胚胎侵袭的体内变化， 导致识别出更好的植入窗口的特异性标记。 出版物： 没有。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Objective: To investigate the endometrial immunomorphological response to procedures mimicking embryo transfer (MET) in rhesus monkey. PROGRESS: Although substantial progress was made in IVF technology in recent decades, the implantation rate remains low. Because it is not possible to conduct direct investigation of embryo invasion in early human pregnancy, the morphophysiological similarity of the reproductive tract makes the rhesus monkey a valuable animal model for the in vivo study of the interactions between the human embryo and endometrium during the implantation process. The study consisted of four animal groups: 1) One animal with tubal and two animals with transcervical MET, using rhesus ES cell-derived embryoid bodies (EB transfer); 2) Two animals with transcervical MET with EB-media only (Sham transfer); 3) Two animals treated with progesterone without MET; and 4) One animal as an untreated control. Animals were euthanized on day 9 or 10 (one monkey on day 21) post transfer. We conducted immunohistochemistry for leukocyte subset and endothelial markers and PAS-staining with vascular and general endometrial morphometry. Immunostaining revealed a decreased number of NK cells (CD56+ and perforin+), CD68+ macrophages and neutrophils in upper layer of endometrium in all experimental groups compared with the untreated control. These changes in leukocyte subsets coincide with decreases in the stromal cell density, increases in the endometrial thickness and vascularity. In the basal endometrium, the number of perforin+ cells increased in all animals, while T cells decreased in the monkeys with MET. Surprisingly, DC-SIGN+ cells were found in the endometrium in most (but not all) animals with MET, although in others groups these cells were absent. We conclude that the rhesus monkey could be considered as a potential model for monitoring in vivo changes in endometrial receptivity and embryo invasion, which may lead to the identification of better specific markers for the window of implantation. PUBLICATIONS: None.