ANALYSIS OF RNA FROM ECTOPIC ENDOMETRIUM IN RHESUS MACAQUES

恒河猴异位子宫内膜 RNA 分析

• 批准号: 8357805
• 负责人: OV D SLAYDEN
• 金额: $ 4.36万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357805
• 关键词: Affinity; Androgen Antagonists; Androgen Receptor; Anti-Progestin; Archives; Bicalutamide; Biological Models; Cell Proliferation; Data; Endometrial; Endometrium; Estrogens; Funding; Gene Expression; Genes; Genome; Goals; Grant; Intra-abdominal; Macaca; Macaca mulatta; Mineralocorticoid Receptor; Modeling; Molecular Analysis; National Center for Research Resources; Preclinical Testing; Primates; Principal Investigator; Progesterone Receptors; RNA analysis; Research; Research Infrastructure; Resources; Sampling; Selective Estrogen Receptor Modulators; Site; Source; Spironolactone; Techniques; Testing; Tissue-Specific Gene Expression; Transplantation; United States National Institutes of Health; cost; endometriosis; eplerenone; novel; subcutaneous

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Macaque endometrium can be auto-transplanted to ectopic intra-abdominal and subcutaneous sites. These endometrial grafts provide an excellent endometriosis-like model system for preclinical testing of novel anti-endometriotic therapies. In this model we have tested the effects of anti-estrogenic selective estrogen receptor modulators (SERMs), spironolactone (anti-mineralocorticoid receptor, anti-MR; anti-progesterone receptor, anti-PR; anti-androgen receptor, anti-AR) with Eplerenone (an anti-MR with no known affinity for AR), Casodex, (a pure anti-AR) and ZK 099 (a pure anti-PR) on estrogen action on ectopic endometrium in rhesus macaques. These samples were analyzed by morphological and immunocytochemical techniques for cell proliferation. The remaining samples arre archived for molecular analysis. Our goal is to analyze gene expression in these archived samples, using the Affymetrix GeneChip¿ Rhesus Macaque Genome Array. The resulting data on differential gene expression will be used to identify genes differentially regulated by these proprietary compounds.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 猕猴子宫内膜可以自体移植到异位的腹腔内和皮下部位。 这些子宫内膜移植物为新型抗子宫内膜异位疗法的临床前试验提供了一个极好的子宫内膜异位样模型系统。 在该模型中，我们测试了抗雌激素选择性雌激素受体调节剂（SERM）、螺内酯（抗盐皮质激素受体，抗MR;抗孕酮受体，抗PR;抗雄激素受体，抗AR）与依普利酮（一种对AR没有已知亲和力的抗MR）、Casodex（一种纯抗AR）和ZK 099（一种纯抗PR）对雌激素作用于恒河猴异位子宫内膜的影响。 通过形态学和免疫细胞化学技术分析这些样品的细胞增殖。其余样品存档用于分子分析。 我们的目标是分析这些存档样本中的基因表达，使用AffysseGeneChip <$Rhesus Macaque基因组阵列。 由此产生的差异基因表达的数据将被用来确定这些专利化合物差异调节的基因。