ANALYSIS OF RNA FROM ECTOPIC ENDOMETRIUM IN RHESUS MACAQUES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 猕猴子宫内膜可以自体移植到异位的腹内和皮下。这些子宫内膜移植物为抗子宫内膜异位症新疗法的临床前测试提供了一个很好的类似子宫内膜异位症的模型系统。在这个模型中，我们测试了抗雌激素选择性雌激素受体调节剂(SERM)、螺内酯(抗盐皮质激素受体，抗MR；抗孕激素受体，抗PR；抗雄激素受体，抗AR)与依普利酮(一种已知与AR没有亲和力的抗MR)、Casodex(一种纯抗AR)和ZK 099(一种纯抗PR)对恒河猴异位内膜雌激素作用的影响。用细胞形态和免疫细胞化学技术进行细胞增殖分析。剩余的样品被存档以进行分子分析。我们的目标是使用Affymetrix基因芯片猕猴基因组阵列来分析这些存档样本中的基因表达。由此产生的差异基因表达数据将用于识别受这些专利化合物差异调控的基因。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Macaque endometrium can be auto-transplanted to ectopic intra-abdominal and subcutaneous sites. These endometrial grafts provide an excellent endometriosis-like model system for preclinical testing of novel anti-endometriotic therapies. In this model we have tested the effects of anti-estrogenic selective estrogen receptor modulators (SERMs), spironolactone (anti-mineralocorticoid receptor, anti-MR; anti-progesterone receptor, anti-PR; anti-androgen receptor, anti-AR) with Eplerenone (an anti-MR with no known affinity for AR), Casodex, (a pure anti-AR) and ZK 099 (a pure anti-PR) on estrogen action on ectopic endometrium in rhesus macaques. These samples were analyzed by morphological and immunocytochemical techniques for cell proliferation. The remaining samples arre archived for molecular analysis. Our goal is to analyze gene expression in these archived samples, using the Affymetrix GeneChip¿ Rhesus Macaque Genome Array. The resulting data on differential gene expression will be used to identify genes differentially regulated by these proprietary compounds.