EVAL OF NORMAL & ECTOPIC IMPLANTATION PRIMATES W CONTRAST ENHANCED ULTRASOUND

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们的目标是对猕猴早期宫内妊娠(IUP)进行研究。在怀孕期间，子宫内膜的血管被广泛修改，为成长中的胚胎提供血液流动。超声造影(CEU)是一种能够对器官内微血管进行高分辨率成像的新技术。该方法依赖于检测充气的微泡，这些微泡在超声成像时被静脉注射。我们的假设是，CEU成像将使胚胎植入的早期可视化成为可能，并首次详细评估胎儿-母体交界处不断生长的子宫内膜血管。我们的具体目标是开发微气泡CEU，用于早期妊娠的可视化和在猕猴胚胎植入期间评估子宫内血管参数。这一目标正在进行中，我们已经对5只恒河猴怀孕早期的子宫内膜进行了成像。我们假设的扩展是微泡CEU可能是研究和早期诊断包括异位妊娠(EP)在内的妊娠疾病的有用工具。EP是一种严重的情况，发育中的胚胎附着并植入子宫外，最常见的是在输卵管内。早期诊断是成功治疗EP的关键，我们计划探索一种新的模型，用于实验诱导猕猴EP。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our goal is to perform studies on early intrauterine pregnancy (IUP) in rhesus macaques. During pregnancy the vessels of the endometrium are extensively modified to provide blood flow to the growing conceptus. Contrast-enhanced ultrasound (CEU) is a new technique capable of high-resolution imaging of the microvasculature within organs. The method relies on the detection of gas-filled microbubbles that are injected intravenously while imaging by ultrasound. Our hypothesis is that CEU imaging will permit earlier visualization of embryo implantation and for the first time a detailed evaluation of the growing endometrial vessels at the fetal-maternal interface. Our Specific Aim is to develop microbubble CEU for visualization of early pregnancy and assessment of intrauterine vascular parameters during embryo implantation in rhesus macaques. This aim is underway and we have imaged the endometrium during early pregnancy in 5 rhesus macaques. An extension of our hypothesis is that microbubble CEU may be a useful tool in the study and early diagnosis of pregnancy disorders including ectopic pregnancy (EP). EP is a serious condition where a developing conceptus attaches and implants outside the uterus, most frequently within the fallopian tube. Early diagnosis is essential for successful treatment of EP, and we plan to explore a new model for experimentally-induced EP in rhesus macaques.