Gene Expression in Nonspecific Orbital Inflammatory Disease

非特异性眼眶炎症性疾病中的基因表达

• 批准号: 8328921
• 负责人: JAMES T ROSENBAUM
• 金额: $ 43.74万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-30 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8328921
• 关键词: Adrenal Cortex Hormones; Biological Assay; Biopsy; Blindness; Clinical; Collection; Decision Making; Diagnosis; Diplopia; Disease; Dose; Fatty acid glycerol esters; Formalin; Gene Expression; Gene Expression Microarray Analysis; Gene Expression Profile; Gene Expression Profiling; Genes; Granulomatous; Hamman-Rich syndrome; Heterogeneity; Histology; Histopathology; Hyperthyroidism; Inflammation; Inflammatory; International; Lacrimal gland structure; Malignant Neoplasms; Methods; Molecular; Morbidity - disease rate; Ocular orbit; Oral; Pain; Paraffin Embedding; Pathogenesis; Pathologist; Patients; Pattern; Pharmaceutical Preparations; Pharmacogenomics; Principal Investigator; Sarcoidosis; Scleroderma; Sclerosis; Statistical Data Interpretation; Surgeon; Techniques; Testing; Therapeutic; Time; Tissues; Vision; Wegener' s Granulomatosis; base; experience; improved; insight; novel; orbit muscle; outcome forecast; prognostic; programs; response; success; thyroid associated ophthalmopathies; tissue culture

DESCRIPTION (provided by applicant): Nonspecific orbital inflammation (NSOI) is an inflammatory disease which causes pain, diplopia, and loss of vision. The pathogenesis of NSOI is largely unknown and virtually unstudied. Microarray analysis of gene expression has enjoyed marked success in providing novel insights into disease pathogenesis. In addition, microarray profiling has been used to identify distinct subsets of disease that in many instances are indistinguishable by histopathology. We propose to utilize microarray gene expression profiling from formalin- fixed, paraffin-embedded (FFPE) orbital biopsies to gain insight into the pathogenesis of NSOI. Gene expression patterns from orbital tissue of patients with NSOI will be compared with gene expression patterns from orbital biopsies from patients with thyroid orbitopathy, sarcoidosis, or Wegener's granulomatosis as well as patterns from normal orbital fat, normal lacrimal gland, or extraocular muscle. We hypothesize that patients with NSOI will have distinct patterns of gene expression that will provide insights into the pathogenesis of the disease. Furthermore, we hypothesize that NSOI is a heterogeneous collection of diseases and that this heterogeneity can be definitively demonstrated by gene expression profiling. Finally we hypothesize that gene expression profiling will help in prognostic and therapeutic decision making for patients with this highly morbid diagnosis.

描述（由申请人提供）： 非特异性眼眶炎症 (NSOI) 是一种导致疼痛、复视和视力丧失的炎症性疾病。 NSOI 的发病机制在很大程度上是未知的，并且几乎未经研究。基因表达的微阵列分析在为疾病发病机制提供新见解方面取得了显着的成功。此外，微阵列分析已用于识别疾病的不同子集，在许多情况下，组织病理学无法区分这些疾病。我们建议利用福尔马林固定石蜡包埋 (FFPE) 眼眶活检的微阵列基因表达谱来深入了解 NSOI 的发病机制。 NSOI 患者眼眶组织的基因表达模式将与甲状腺眼病、结节病或韦格纳肉芽肿患者眼眶活检的基因表达模式以及正常眼眶脂肪、正常泪腺或眼外肌的基因表达模式进行比较。我们假设 NSOI 患者具有独特的基因表达模式，这将有助于深入了解该疾病的发病机制。此外，我们假设 NSOI 是疾病的异质性集合，并且这种异质性可以通过基因表达谱明确证明。最后，我们假设基因表达谱将有助于患有这种高度病态诊断的患者的预后和治疗决策。