The Interplay Between the Circadian Clock and Plant Immune Mechanisms

昼夜节律钟与植物免疫机制之间的相互作用

• 批准号: 8368842
• 负责人: Xinnian Dong
• 金额: $ 29.07万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-17 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8368842
• 关键词: Affect; Agriculture; Animals; Apoptosis; Arabidopsis; Autoimmune Diseases; Behavior; Binding; Biological Process; Biological Response Modifiers; Brain; Brassicaceae; Cell Survival; Cells; Circadian Rhythms; Cluster Analysis; Complex; Data; Defense Mechanisms; Detection; Disease; Disease Resistance; Dissection; Employee Strikes; Environment; Equilibrium; Eukaryota; Famines; Gene Expression; Genes; Genetic; Genetic Epistasis; Genetic Transcription; Health; Human; Immune; Immune response; Immunity; Infection; Interleukin-1 Receptors; Jet Lag Syndrome; Knock-out; Leucine-Rich Repeat; Life; Link; Mediating; Metabolism; Molecular; Molecular Genetics; Molecular Profiling; Movement; Mutate; Natural Immunity; Nuclear Translocation; Nucleotides; Obesity; Oomycetes; Organ; Organism; Oxidation-Reduction; Pattern; Peripheral; Pesticides; Physiologic pulse; Phytophthora; Plant Leaves; Plants; Population; Potato; Predisposition; Production; Proteins; Regulation; Relative (related person); Reporter; Reporting; Reproduction spores; Resistance; Resources; Role; Salicylic Acids; Signal Transduction; Site; System; Testing; Time; Tissues; Whole Organism; base; circadian pacemaker; cofactor; design; fitness; functional group; mathematical model; mutant; overexpression; pathogen; promoter; response; vpr Genes

DESCRIPTION (provided by applicant): The circadian clock has profound impacts on living organisms on earth. It maximizes the fitness of an organism by enabling it to coordinate metabolism and behavior with its environment. However, studies that unequivocally demonstrate the fitness benefits are scarce and the underlying molecular mechanisms are poorly known. The surprising discovery that the Arabidopsis clock component, CCA1, is a key regulator of RPP4-mediated resistance against the oomycete pathogen Hyaloperonospora arabidopsidis (Hpa) leads to this first in depth study of the interplay between the circadian clock and plant immunity mechanisms. Aim 1 of this study will focus on the dissection of the regulatory circuitry between CCA1 and the resistance protein RPP4 using molecular genetics and systems approaches. A mathematical model will be made to explain how RPP4 perturbs CCA1-mediated rhythmic expression of defense genes resulting in programmed cell death of the infected cell and resistance to Hpa. Aim 2 will investigate the role of the circadian clock in systemic acquired resistance to test the hypothesis that the clock is reset during this immune response to redirect the resource to defense and promote cell survival. There are striking parallels between plant defense mechanisms and animal innate immunity. The influence of the circadian clock on animal immune responses has also been reported. However, in depth studies are challenging because of the inherent complexities of animal circadian clocks (central and peripheral) and of the microflora. Therefore, the proposed Arabidopsis-Hyaloperospora study will have a great advantage in revealing the design principles underline the interplay between the circadian clock and immunity besides providing detailed molecular mechanisms. The system perspectives on plants immune mechanisms will help us design better strategies to control crop disease and reduced the use of pesticides, which are hazardous to the environment and human health. Plant health has always had a significant impact on human health as demonstrated by the infamous Irish potato famine in 1840s, which was caused by Phytophthora infestans, a close relative of Hpa used in this study. With the world population exploding, developing better and safer agricultural practices is not a choice, but a necessity. PUBLIC HEALTH RELEVANCE: The circadian clock affects many aspects of human lives including jetlag, obesity, and immunity. However, the molecular mechanisms are poorly understood because humans have the complex central clock in the brain as well as peripheral clocks in different organs. This project will use a simpler organism, thale cress, to study how the circadian controls the timing of plant immune responses against infection and develop new ways to control crop disease and reduce the use of pesticides hazardous to human health and environment.

描述（由申请人提供）：生物钟对地球上的生物体有着深远的影响。它通过使生物体的新陈代谢和行为与环境协调来最大限度地提高生物体的适应性。然而，明确证明健身益处的研究很少，并且对潜在的分子机制知之甚少。令人惊讶的发现是，拟南芥时钟组件，CCA 1，是RPP 4介导的抗卵菌病原体透明operonospora arabidopsidis（Hpa）抗性的关键调节因子，这导致了首次深入研究生物钟之间的相互作用 和植物免疫机制。本研究的目的1将集中在使用分子遗传学和系统方法的CCA 1和抗性蛋白RPP 4之间的调控回路的解剖。将建立一个数学模型来解释RPP 4如何干扰CCA 1介导的防御基因的节律性表达，从而导致受感染细胞的程序性细胞死亡和对Hpa的抗性。目的2将研究生物钟在系统获得性抵抗中的作用，以检验生物钟在这种免疫应答期间重置以将资源重新定向到防御和促进细胞存活的假设。植物的防御机制和动物的先天免疫之间有着惊人的相似之处。生物钟对动物免疫反应的影响也有报道。然而，由于动物生物钟（中枢和外周）和微生物群落的固有复杂性，深入研究具有挑战性。因此，拟进行的白葡萄球菌-透明operospora研究将具有很大的优势，除了提供详细的分子机制外，还可以揭示生物钟和免疫之间相互作用的设计原则。植物免疫机制的系统观点将有助于我们设计更好的策略来控制作物病害，减少对环境和人类健康有害的农药的使用。植物健康一直对人类健康产生重大影响，正如19世纪40年代臭名昭着的爱尔兰马铃薯饥荒所证明的那样，这是由本研究中使用的Hpa的近亲Phytophthora infestans引起的。随着世界人口的激增，发展更好、更安全的农业实践不是一种选择，而是一种必要。 生物钟影响人类生活的许多方面，包括时差，肥胖和免疫力。然而，分子机制知之甚少，因为人类大脑中有复杂的中央时钟，不同器官中也有外围时钟。这个项目将使用一种更简单的有机体，塔勒水芹，来研究 昼夜节律控制植物对感染的免疫反应的时间，并开发新的方法来控制作物病害，减少对人类健康和环境有害的农药的使用。