Effect of Age on Antibody Diversity

年龄对抗体多样性的影响

• 批准号: 8552476
• 负责人: PATRICIA J GEARHART
• 金额: $ 45.39万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8552476
• 关键词: Affect; Affinity; Age; Aging; Antibody Diversity; Antigens; B-Lymphocyte Subsets; B-Lymphocytes; Cells; Chromosomal translocation; Chronic; DNA Repair; Frequencies; Human; IgG Receptors; Immunoglobulin Class Switching; Immunoglobulin Somatic Hypermutation; Immunoglobulin Switch Recombination; Lymphoma; Malignant Neoplasms; Measures; Mus; Mutation; Production; age effect; aged; exhaust; immune function; repair enzyme

We will see if ABCs have encountered antigen and undergone somatic hypermutation and class switching. Diversity in the repertoire and the mutation frequency will be examined in old mice. This may determine if the old cells are exhausted from chronic antigen exposure. We will see if DNA repair affects the production of ABCs by using mice deficient for repair enzymes. We will also measure the frequency of chromosome translocations in these cells, to study the mechanism of how aging produces cancer.

我们将看看 ABC 是否遇到了抗原并经历了体细胞超突变和类别转换。将在老年小鼠中检查库的多样性和突变频率。 这可以确定老细胞是否因长期接触抗原而疲惫不堪。我们将通过使用缺乏修复酶的小鼠来观察 DNA 修复是否会影响 ABC 的产生。我们还将测量这些细胞中染色体易位的频率，以研究衰老如何产生癌症的机制。