Effect of Age on Antibody Diversity

年龄对抗体多样性的影响

• 批准号: 8931596
• 负责人: PATRICIA J GEARHART
• 金额: $ 102.41万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8931596
• 关键词: Affinity; Age; Antibody Diversity; Antigens; B-Lymphocyte Subsets; B-Lymphocytes; Cells; Chronic; Clone Cells; Frequencies; Human; IgG Receptors; Immunoglobulin Class Switching; Immunoglobulin Somatic Hypermutation; Immunoglobulin Switch Recombination; Light; Lymphoma; Measures; Mus; Mutation; Testing; age effect; aged; exhaust; expression vector; immune function; prevent

We will see if ABCs have encountered antigen and undergone somatic hypermutation and class switching. Diversity in the repertoire and the mutation frequency will be examined in old mice. This may determine if the old cells are exhausted from chronic antigen exposure. To determine what antigens induce non-responsive B cells, we will sequence heavy and light chains from single cells, clone them into expression vectors, and test for reactivity to a panel of antigens. To see if ABCs prevent affinity maturation of follicular cells, we will transfer cells into recipient mice, immunize, and measure affinity.

我们将观察ABC是否遇到抗原并经历了体细胞超突变和类别转换。将在老年小鼠中检查库的多样性和突变频率。 这可以确定旧细胞是否因慢性抗原暴露而耗尽。为了确定什么抗原诱导无应答B细胞，我们将对来自单个细胞的重链和轻链进行测序，将它们克隆到表达载体中，并测试对一组抗原的反应性。为了观察ABC是否阻止滤泡细胞的亲和力成熟，我们将细胞转移到受体小鼠中，免疫并测量亲和力。