Mechanisms of Visual Transduction

视觉传导机制

基本信息

批准号：
8327970
负责人：
Edward N Pugh
金额：
$ 18.9万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
1978
资助国家：
美国
起止时间：
1978-08-01 至 2016-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8327970
关键词：
11 cis Retinal Accounting Address Affect Affinity Anabolism Behavioral Calcium Cells Cellular biology Cyclic GMP Data Dependence Foundations Future GTP-Binding Proteins Gene Expression Regulation Generations Guanylate Cyclase Human Knockout Mice Lead Life Light Light Adaptations Mammals Mannosidase Membrane Metabolism Methods Molecular Mus Opsin Photoreceptors Phototransduction Physiologic pulse Physiological Pigments Point Mutation Predisposition Property Proteins Regulation Research Retinal Retinal Cone Retinal Diseases Retinitis Pigmentosa Retinoids Rhodopsin Role Signal Transduction Source Testing Time Transcript Translating Vertebrate Photoreceptors Vision Visual Visual system structure Work base behavior test chromophore insight light intensity man novel operation protein folding protein misfolding recoverin protein retinal rods trafficking ubiquitin-protein ligase visual cycle

项目摘要

DESCRIPTION (provided by applicant): Cone photoreceptor cells initiate vision in daytime, operating in light intensities that saturate rods and render them useless to the visual system. The ability of cones to escape saturation relies in large part upon specializations of proteins, including cone opsins, within the cone phototransduction cascade that function in synergy to preserve inward cGMP-sensitive current in strong light. During their biosynthesis, cone opsins have a surprising need for the visual chromophore, 11-cis retinal, to achieve proper folding, and our preliminary data suggests that they also possess a compensating capacity for proteolytic degradation of misfolded proteins. The proposed work will lead to a comprehensive, molecularly based account of these specializations within cones, and could be used in future work to both extend the operating range of the much more numerous rods, and develop strategies that facilitate the generation of properly folded opsins or increase the capacity to degrade misfolded opsins.

描述（由申请人提供）：锥形光感受器细胞在白天启动视觉，以光强度运行，使杆饱和并使它们对视觉系统无用。锥体逃脱饱和度的能力在很大程度上取决于蛋白质（包括锥锥）在锥光传输级联反传递中的蛋白质（包括锥孔）的能力，可在协同作用中起作用，以在强光中保存向内的CGMP敏感电流。在其生物合成期间，锥蛋白具有令人惊讶的对视觉发色团（11 cis视网膜）的需求，以实现适当的折叠，我们的初步数据表明，它们还具有蛋白水解降解错误折叠蛋白的补偿能力。拟议的工作将导致对锥体内的这些专业化的全面，分子的说明，并可以在将来的工作中使用，以扩展更多杆的工作范围，并制定策略，以促进正确折叠的Opsins的生成或增加降解错误折叠的OPSIS的能力。