Genomics Core Facility

基因组学核心设施

• 批准号: 8554138
• 负责人: Michael Krause
• 金额: $ 384.83万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8554138
• 关键词: Adopted; Back; Bioinformatics; Cells; Core Facility; Data; Data Analyses; Data Quality; Databases; Deposition; Diabetes Mellitus; Digestive System Disorders; Disease; Ensure; Gastrointestinal Diseases; Gene Expression; Genomics; Goals; Image; Individual; Institutes; Kidney Diseases; Liver diseases; Metabolic Diseases; Mission; National Institute of Diabetes and Digestive and Kidney Diseases; Procedures; Process; Quality Control; Recovery; Reproducibility; Research Personnel; Sampling; Scientist; Services; Site; Text; Time; United States National Institutes of Health; cost; instrument; interest; member; next generation

The Genomics Core Facility is a high quality microarray and next generation sequencing processing site that can be accessed by any NIDDK P.I. for gene expression analysis related to diseases of programmatic interest, including diabetes, metabolic disorders, and liver and gastrointestinal disease. Time permitting, our microarray services are available to other NIH ICs. For gene expression, the Core Staff acquires various sample RNAs from investigators, checks sample quality, makes probes, hybridizes and washes expression arrays, collects data, and deposits all data in a central database that is accessible to DDK staff. Our bioinformatician also provides basic data analysis to all customers to ensure data quality. Additional analysis is provided as requested when feasible. For efficiency and reproducibility we have adopted a single array platform. We also provide high capacity, parallel sequencing services using the Illumina platform. We generate or acquire size-selected samples from researchers, generate flow cell clusters, and capture image/sequence data. That data is then converted to text files of short tag (26-101nt) sequence information and passed back to individual investigators for analysis. We provide limited bioinformatic support for this data at this time with the bulk of the burden for analysis being placed on the individual investigators. This service is open to all DDK P.I.s.

基因组学核心设施是一个高质量的微阵列和下一代测序处理站点，可以被任何NIDDK pi访问，用于与程序感兴趣的疾病相关的基因表达分析，包括糖尿病，代谢紊乱，肝脏和胃肠道疾病。时间允许，我们的微阵列服务可用于其他NIH ic。