Role of Gq/11-alpha in metabolic regulation

Gq/11-alpha 在代谢调节中的作用

• 批准号: 8349964
• 负责人: Lee Weinstein
• 金额: $ 19.89万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8349964
• 关键词: Alleles; Eating; Energy Metabolism; Environment; Enzymes; GTP-Binding Proteins; Genes; Goals; Hormones; House mice; Hypothalamic structure; Life; Metabolic; Morbid Obesity; Mus; Neuraxis; Neurotransmitters; Peripheral; Phenotype; Phospholipase C; Play; Regulation; Role; Signal Transduction; Tissues; Transgenes; Transgenic Mice; Underweight; Weight Gain; extracellular; glucose metabolism; paraventricular nucleus; promoter; receptor coupling; recombinase

To date we have generated mice with Gq/11-alpha deficiency in the paraventricular nucleus of the hypothalamus (PVN) using Sim1-cre transgenic mice. Preliminary findings show that these mice are underweight in the first month of life but then do develop severe obesity. However when the mice are housed individually they do not gain weight, suggesting that environment may imppact on the phenotype. Studies are underway to determine to what extent this is due to excess food intake vs. reduced energy expenditure.

到目前为止，我们已经产生了Gq/11-α缺陷的下丘脑室旁核（PVN）使用Sim 1-cre转基因小鼠。初步研究结果表明，这些小鼠在出生后的第一个月体重不足，但随后会发展为严重的肥胖症。然而，当小鼠单独饲养时，它们不会增加体重，这表明环境可能会影响表型。研究正在进行中，以确定这在多大程度上是由于过量的食物摄入与减少的能量消耗。