ECOLOGICAL GENOMICS: NICHE CHARACTERIZATION OF BOECHERA STRICTA

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 需要在自然环境中进行更多的研究，以真正了解其背后的机制 表达模式，并确定这是否是由于特定的适应性性状的遗传。的 实验室实验和实地研究结果的不一致说明了跨学科研究的必要性。 自然环境中的研究。 我们建立了一个共同的花园的近交系和母系家庭的Boechera stricta内和外， 自然发生的补丁，以评估已知的压力反应途径和候选性状的作用（防御 化合物和水分利用效率），以确定生态位的边界，并估计其进化潜力。B。 stricta是拟南芥的另一个野生近亲，使基因组工具如微阵列的使用成为可能。 转录谱分析（见下文方法）。因此，我们正在结合数量遗传学的工具， 生理生态学，化学生态学和基因组学在这个领域的实验，以了解问题的生态位， 对进化生态学家来说至关重要。 2010年夏天，西门子博士接待了一位来自USF的研究员。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The need for more studies in natural environmental settings is required to truly understand the mechanisms behind expression patterns of a population and to determine if this is due to the inheritance of specific adaptive traits. The inconsistency of results between laboratory experiments and field studies illustrates the need for interdisciplinary studies in natural environments. We established a common garden of inbred lines and maternal families of Boechera stricta within and just outside a naturally occurring patch to evaluate the role of known stress response pathways and candidate traits (defensive compounds and water use efficiency) in determining niche boundaries and to estimate their evolutionary potential. B. stricta is another close wild relative of Arabidopsis, enabling the use of genomic tools such as microarrays for genomewide transcript profiling (see below under Methods). Thus we are combining the tools of quantitative genetics, physiological ecology, chemical ecology and genomics in this field experiment to understand issues of the niche that are fundamentally important to evolutionary ecologists. Dr. Siemens hosted a fellow from USF during the summer of 2010.