ISOLATION AND STRUCTURAL CHARACTERIZATION OF MARINE FUNGAL SIDEROPHORES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 背景：许多微生物产生低分子量的铁（III）特异性结合化合物，称为铁载体，以竞争铁。微生物被认为产生独特的铁载体结构，以成功地与环境中的其他生物竞争铁。从陆地细菌和真菌以及海洋细菌中分离的铁载体显示出作为生物医学试剂和控制微生物生长的前景。由海洋真菌产生的铁载体尚未被表征，因此提供了新的生物活性化合物的未开发的来源。 假设：海洋来源的真菌产生具有新结构的铁载体。 方法：筛选海洋来源的真菌菌株，在铁限制条件下在纯培养物中生产铁载体。分离铁载体，并使用氨基酸分析、脂肪酸分析、NMR、质谱法和其他技术（视情况而定）进行结构表征。 成果和未来方向：已筛选出12种海洋真菌菌株的铁载体生产，12个中的11个被发现产生铁载体。目前正在对三种菌株产生的铁载体进行结构分析。从南极的一株白孢子虫中分离到一种分泌型铁载体。从Sporidiobolus salmonicolor 05-001中分离到一种分泌型异羟肟酸铁载体和两种细胞结合型铁载体。从莱斯特青霉ATCC 42470中分离到一种分泌型铁载体。这些铁载体的质谱和核磁共振分析已经完成。目前的工作重点是基于NMR和质谱数据的结构测定。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Background: Many microorganisms produce low-molecular-weight, iron(III) specific binding compounds called siderophores to compete for iron. Microbes are thought to produce unique siderophore structures to successfully compete for iron against other organisms in their environment. The siderophores isolated from terrestrial bacteria and fungi and from marine bacteria show promise as biomedical agents and for control of microbial growth. The siderophores produced by marine fungi have not been characterized and thus provide an unexplored source of novel bioactive compounds. Hypothesis: Marine-derived fungi produce siderophores with novel structures. Methods: Marine-derived fungal strains are screened for production of siderophores in pure cultures under conditions of iron limitation. Siderophores are isolated and structurally characterized using amino acid analysis, fatty acid analysis, NMR, mass spectrometry, and other techniques, as appropriate. Results and future directions: Twelve marine fungal strains have been screened for siderophore production; eleven of the twelve were found to produce siderophores. Structural analysis of siderophores produced by three strains is currently underway. One secreted siderophore has been isolated from an Antarctic strain of Leucosporidium. One secreted hydroxamate siderophore and two cell-associated siderophores have been isolated from Sporidiobolus salmonicolor 05-001. One secreted siderophore was isolated from Penicillium raistrickii ATCC42470. Mass spectrometric and NMR analyses of these siderophores has been completed. Current work focuses on structure determination based on NMR and mass spectrometric data.