NOVEL SIDEROPHORES PRODUCED BY MARINE BACTERIA AND FUNGI

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 背景：许多微生物产生低分子量的铁（III）特异性结合化合物，称为铁载体，以竞争铁。 微生物被认为产生独特的铁载体结构，以成功地与环境中的其他生物竞争铁。 从陆地细菌和真菌以及海洋细菌中分离的铁载体显示出作为生物医学试剂和控制微生物生长的前景。 由海洋真菌产生的铁载体尚未被表征，因此提供了新的生物活性化合物的未开发的来源。 假设：海洋来源的真菌产生具有新结构的铁载体。 方法：筛选海洋来源的真菌菌株，在铁限制条件下在纯培养物中生产铁载体。 分离铁载体，并使用氨基酸分析、脂肪酸分析、NMR、质谱法和其他技术（视情况而定）进行结构表征。 结果和未来的方向：在这个时候，我们已经确定Cunninghamella elegans ATCC 36112作为铁载体生产海洋来源的真菌。 一种新的铁载体已被分离出的真菌，其特征在于使用紫外可见分光光度法，电喷雾质谱，串联质谱。 我们目前正在努力纯化足够的材料进行核磁共振分析，这将使我们能够完成这种新型铁载体的结构测定。 我们还开始筛选几种海洋来源的酵母菌株。 这些菌株似乎产生铁载体（基于Fe（III）-CAS测定），我们将立即开始纯化化合物。 计划在不久的将来进行的实验将分离这些新菌株产生的铁载体并对其结构进行表征。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Many microorganisms produce low-molecular-weight, iron(III)-specific binding compounds called siderophores to compete for iron. Microbes are thought to produce unique siderophore structures to successfully compete for iron against other organisms in their environment. The siderophores isolated from terrestrial bacteria and fungi and from marine bacteria show promise as biomedical agents and for control of microbial growth. The siderophores produced by marine fungi have not been characterized and thus provide an unexplored source of novel bioactive compounds. Hypothesis: Marine-derived fungi produce siderophores with novel structures. Methods: Marine-derived fungal strains are screened for production of siderophores in pure cultures under conditions of iron limitation. Siderophores are isolated and structurally characterized using amino acid analysis, fatty acid analysis, NMR, mass spectrometry, and other techniques, as appropriate. Results and future directions: At this time, we have identified Cunninghamella elegans ATCC 36112 as a siderophore producing marine-derived fungus. A novel siderophore has been isolated from the fungus and characterized using UV-Visible spectrophotometry, electrospray ionization mass spectrometry, and tandem mass spectrometry. We are currently working to purify sufficient material for analysis by NMR, which will allow us to complete the structural determination for this novel siderophore. We have also begun screening several strains of marine-derived yeasts. These strains appear to produce siderophores (based on Fe(III)-CAS assay) and we will begin purification of the compounds immediately. Experiments planned in the immediate future will isolate and structurally characterize siderophores produced by these new strains.