HIGH-RESOLUTION EX VIVO IMAGING OF AN ALZHEIMER'S MOUSE MODEL

阿尔茨海默病小鼠模型的高分辨率离体成像

• 批准号: 8363147
• 负责人: JOANNA L JANKOWSKY
• 金额: $ 1.23万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363147
• 关键词: Alzheimer' s Disease; Amyloid deposition; Amyloidosis; Animals; Area; Atlases; Brain; Collaborations; Complement; Data; Data Analyses; Disease; Funding; Gene Expression; Goals; Grant; Image; Magnetic Resonance Imaging; Microscopy; Molecular Profiling; Mus; National Center for Research Resources; Neurodegenerative Disorders; Principal Investigator; Research; Research Infrastructure; Resolution; Resources; Source; Testing; Tetanus Helper Peptide; Time; United States National Institutes of Health; Work; cost; mouse model

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Using MR 9T magnet for this study. The MouseBIRN collaborative group seeks testbed cases to generate a searchable, online atlas that combines gene expression and 3D MRI images of mouse models for neurodegenerative disease. We would like to develop the new tet-off APP mouse model of Alzheimer's disease in this context. Work is already under way to generate gene expression profiles of the tet-off APP mice at multiple points in the progression of amyloidosis. The imaging proposed in collaboration with CIVM would provide comprehensive, high-resolution, 3D morphological images to complement the gene expression analyses. We will focus imaging efforts on two of the five time points assessed for gene expression, one before and one after the onset of amyloid deposition. The goal is to develop an average rendering from 5 Alzheimer's (tet-off APP) and 5 control (tTA) animals at each time point that can be used not only as a test of the MouseBIRN automated data analysis pipeline but also provide valuable information about volumetric and/or structural changes in specific brain areas as the disease progresses. Data from this study will be made publicly available through the MouseBIRN network.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 本研究使用MR 9 T磁体。MouseBIRN协作组寻求试验台案例，以生成一个可搜索的在线图谱，该图谱结合了神经退行性疾病小鼠模型的基因表达和3D MRI图像。 我们希望在这种情况下开发新的tet-off APP小鼠阿尔茨海默病模型。 研究人员已经开始在淀粉样变性进展的多个点上生成tet-off APP小鼠的基因表达谱。 与CIVM合作提出的成像将提供全面的，高分辨率的3D形态学图像，以补充基因表达分析。 我们将把成像工作集中在五个时间点中的两个，一个在淀粉样蛋白沉积开始之前，一个在淀粉样蛋白沉积开始之后。 目标是在每个时间点从5只阿尔茨海默氏症（tet-off APP）和5只对照（tTA）动物中开发平均渲染，其不仅可用作MouseBIRN自动数据分析管道的测试，而且还提供关于随着疾病进展特定脑区的体积和/或结构变化的有价值信息。本研究的数据将通过MouseBIRN网络公开提供。