13C-LABELING FOR IMPROVED STRUCTURE DETERMINATION

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 使用13标记的葡萄糖，我标记了一种化合物(来自细菌来源)，具有非常高的掺入率(90%)。这种标记允许使用13C-13C COSY实验利用碳-碳连接性来确定化合物的结构。然而，我正在尝试确定用13C-13C COSY确定结构所需的最低化合物数量。使用冷冻探头，所需化合物的量应该很少。这一少量的化合物将允许快速确定化合物的结构，即使只有很少量的化合物可以获得，这是天然产品领域研究的关键。这一工具可以极大地推动天然产物结构测定领域的发展。在研究了13C-13C COSY之后，我正在研究一台自然丰度为13C的DQCOSY。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Using 13-labeled glucose, I have labeled a compound (from a bacterial source) with very high incorporation (90%). This labeling has allowed for the structure determination of the compound with the use of carbon-carbon connectivity using the 13C-13C COSY experiment. However, I am attempting to determine the minimum amount of compound necessary to determine the structure with a 13C-13C COSY. With a cryoprobe, the amount of compound needed should be very small. This small amount will allow for the rapid structure determination of compounds when even a very little amount of compound is attainable, a key to research in the field of natural products. This tool could advance the field of structure determination in natural products tremendously. After working on the 13C-13C COSY, I am working on a DQCOSY at natural abundance for 13C.