Omics approaches for natural products discovery

天然产物发现的组学方法

• 批准号: 8793792
• 负责人: TIMOTHY S BUGNI
• 金额: $ 28.28万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-01 至 2016-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8793792
• 关键词: Antibiotics; Area; Bacteria; Biodiversity; Biological Factors; Chemicals; Chemistry; Coculture Techniques; Communicable Diseases; Communication; Data; Detection; Drug Design; Drug usage; Environment; FDA approved; Florida; Fractionation; Gene Expression Regulation; Generations; Genomics; Goals; Habitats; Health; Human; Laboratories; Lead; Libraries; Marine Invertebrates; Marines; Methods; Mono-S; Organism; Outcome; Pharmaceutical Preparations; Planets; Porifera; Production; Property; Proteomics; Puerto Rico; Regulation; Resources; Source; Structure; System; Systems Biology; Technology; Testing; Therapeutic; base; cost effective; design; drug discovery; drug production; genome sequencing; high throughput screening; improved; innovation; metabolomics; microorganism; novel; novel strategies; oncology; screening; small molecule; tool; transcriptomics; tropical coastal water

DESCRIPTION (provided by applicant): The proposal aims to develop new approaches to natural products discovery. While much effort has been placed on designing drug-like synthetic screening libraries, limited efforts have been put forth to logically design natural product screening libraries in terms of improving novelty of hits and increasing drug-like or lead-like structures within the library. This project will evaluate logical natural product library design on the outcome of high-throughput screening, primarily in the areas of oncology and infectious disease. In particular, we will apply LCMS-based untargeted metabolomics to help guide library design. We will use metabolomics, genomics, transcriptomics, and proteomics to investigate interspecies interactions. Understanding the systems biology governing interspecies interactions and secondary metabolite production will uncover new mechanisms to activate cryptic clusters. The outcomes of this proposal will be: (i) a template for producing natural product libraries enriched in new/novel small molecules with drug-like properties; and (ii) a platform for rapidly determining organisms where interspecies interactions change secondary metabolite production.

描述（由申请人提供）：该提案旨在开发天然产物发现的新方法。虽然在设计类药物合成筛选文库方面已经付出了很多努力，但在提高新药的新颖性和增加文库中类药物或类铅结构方面，在逻辑上设计天然产物筛选文库方面的努力有限。本项目将对逻辑自然产物库设计进行评估