INVESTIGATION OF DYNAMICS OF HEADPIECE PROTEINS USING NMR RELAXATION EXPERIMENTS

使用 NMR 弛豫实验研究头件蛋白质的动力学

• 批准号: 8361157
• 负责人: Liliya Vugmeyster
• 金额: $ 0.54万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361157
• 关键词: Biological Process; C-terminal; Chemicals; Chickens; Family; Funding; Grant; Human; Investigation; Modeling; National Center for Research Resources; Principal Investigator; Protein Dynamics; Proteins; Relaxation; Research; Research Infrastructure; Resources; Series; Source; United States National Institutes of Health; cost; quantum; research study; villin

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Studies of protein dynamics are important for understanding various biological functions of proteins. This project involves a series of auto and cross-correlation experiments to obtain a detailed dynamics information of several small model proteins. Specifically, we will look at the fragments of N and C-terminal domains of headpice family: human dematin and chicken villin. Experiments will comprize 15N t1, t2, and heteronuclear NOE, as well as 13C t1, 13C transverse C'-C'CA, C'-C'N, and multiple quantum chemical exchange C'-N.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 蛋白质动力学的研究对于理解蛋白质的各种生物学功能是重要的。本课题通过一系列的自相关和互相关实验，获得了几种小分子模型蛋白质的详细动力学信息。具体而言，我们将研究headpice家族的N和C-末端结构域片段：人dematin和鸡绒毛蛋白。实验将验证15 N t1、t2和杂原子NOE，以及13 C t1、13 C横向C '-C' CA、C '-C' N和多重量子化学交换C '-N。